Programa de Pós-graduação em Neurociências, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Universidade Federal de Minas Gerais, Belo Horizonte 31270-010, Brazil.
Behav Brain Res. 2012 Jul 1;232(2):421-5. doi: 10.1016/j.bbr.2012.04.004. Epub 2012 Apr 9.
The links between spatial behavior and hippocampal levels of synapsin I and phosphosynapsin I were assessed in normal rats and in the pyrithiamine-induced thiamine deficiency (PTD) rat model of Wernicke-Korsakoff's syndrome. Synapsin I tethers small synaptic vesicles to the actin cytoskeleton in a phosphorylation-dependent manner, is involved in neurotransmitter release and has been implicated in hippocampal-dependent learning. Positive correlations between spontaneous alternation behavior and hippocampal levels of both synapsin I and phosphorylated synapsin I were found in control rats. However, spontaneous alternation performance was impaired in PTD rats and was accompanied by a significant reduction (30%) in phosphorylated synapsin I. Furthermore, no correlations were observed between either form of synapsin I and behavior in PTD rats. These data suggest that successful spontaneous alternation performance is related to high levels of hippocampal synapsin I and phosphorylated synapsin I. These results not only support the previous findings that implicate impaired hippocampal neurotransmission in the spatial learning and memory deficits associated with thiamine deficiency, but also suggest a presynaptic mechanism.
空间行为与海马突触素 I 和磷酸化突触素 I 水平之间的联系在正常大鼠和吡哆醇诱导的硫胺素缺乏（PTD）大鼠威尼克 - 科萨科夫综合征模型中进行了评估。突触素 I 以磷酸化依赖的方式将小突触小泡固定在肌动蛋白细胞骨架上，参与神经递质的释放，并与海马依赖性学习有关。在对照组大鼠中发现自发交替行为与海马突触素 I 和磷酸化突触素 I 水平之间存在正相关。然而，PTD 大鼠的自发交替表现受损，磷酸化突触素 I 显著减少（30%）。此外，在 PTD 大鼠中，两种形式的突触素 I 与行为之间均未观察到相关性。这些数据表明，成功的自发交替表现与海马突触素 I 和磷酸化突触素 I 的高水平有关。这些结果不仅支持了先前的发现，即表明海马神经传递受损与硫胺素缺乏相关的空间学习和记忆缺陷有关，还表明存在突触前机制。
