利用交叉引物扩增快速现场检测酸噬果胶杆菌。

Rapid on-site detection of Acidovorax citrulli by cross-priming amplification.

机构信息

College of Plant Protection, Nanjing Agricultural University, China.

出版信息

Mol Cell Probes. 2012 Aug;26(4):175-6. doi: 10.1016/j.mcp.2012.03.010. Epub 2012 Apr 7.

DOI:10.1016/j.mcp.2012.03.010

Abstract

Cross-priming amplification (CPA) for Acidovorax citrulli detection was evaluated in this study. The sensitivity of CPA assay for pure bacterial culture was 3.7 × 10(3) CFU/ml. Bacteria on naturally infected watermelon seeds were detected using CPA assay, suggesting this method is suitable for A. citrulli on-site detection from watermelon seeds.

摘要

本研究评估了瓜类坏死黄单胞菌（Acidovorax citrulli）的交叉引物扩增（CPA）检测方法。CPA 检测方法对纯细菌培养物的灵敏度为 3.7×10(3) CFU/ml。利用 CPA 检测方法检测到了自然感染西瓜种子上的细菌，表明该方法适用于从西瓜种子现场检测 A. citrulli。