利用金标记 DNA 条带传感器快速现场检测西瓜嗜酸菌亚种。
Rapid on-site detection of Acidovorax avenae subsp. citrulli by gold-labeled DNA strip sensor.
机构信息
Chinese Academy of Inspection and Quarantine, Beijing 100029, PR China.
出版信息
Biosens Bioelectron. 2011 Jun 15;26(10):4241-4. doi: 10.1016/j.bios.2011.04.004. Epub 2011 Apr 12.
Acidovorax avenae subsp. citrulli (AAC) is one of the most harmful diseases in cucurbit production. A rapid and sensitive DNA strip sensor was constructed based on gold nanoparticle-labeled oligonucleotide probes for the detection of AAC. Both the qualitative and semi-quantitative detections of target DNA were successfully achieved using the developed DNA strip sensor. The qualitative limit of detection (LOD) of the strip sensor was determined as 4 nM. The LOD for the semi-quantitative detection was calculated to be 0.48 nM in the range of 0-10 nM. The genomic DNA was detected directly using the DNA strip sensor without any further treatment. This DNA strip sensor is a potentially useful tool for rapid on-site DNA screening.
酸噬草酸杆菌亚种柑橘致病变种（AAC）是葫芦科生产中最具危害性的病害之一。本研究构建了一种基于金纳米粒子标记寡核苷酸探针的快速灵敏 DNA 条带传感器，用于检测 AAC。该 DNA 条带传感器可成功实现对目标 DNA 的定性和半定量检测。条带传感器的定性检测限（LOD）确定为 4 nM。在 0-10 nM 的范围内，半定量检测的 LOD 计算为 0.48 nM。该 DNA 条带传感器可直接用于检测基因组 DNA，无需进一步处理。该 DNA 条带传感器是一种用于快速现场 DNA 筛选的潜在有用工具。
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