Environmental Biophysical Chemistry, IIE-ENAC, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 2, CH-1015 Lausanne, Switzerland.
Bioresour Technol. 2012 Jun;114:603-9. doi: 10.1016/j.biortech.2012.03.071. Epub 2012 Mar 29.
The influence of the extraction methods on the composition, size diversity, molar mass and size distributions of exopolymeric substances (EPS) from the bacterium Sinorhizobium meliloti wild type (WT) and by the exoY strain deficient in exopolysaccharide production was investigated. EPS obtained by centrifugation, EDTA and formaldehyde/NaOH were compared. It was found that the extraction method influenced TOC, TN and total protein content in EPS from both strains. However, no difference between EDTA and formaldehyde/NaOH methods was observed for the exopolysaccharide components. Similar functional groups and fluorescence pattern were found in the EPS obtained by different methods; however their relative abundance was method dependent. The extraction method also affected the molar mass and size distribution, HP SEC diversity among different treatment and bacterial strains.
研究了提取方法对苜蓿中华根瘤菌野生型（WT）和 exoY 缺失菌株中胞外聚合物（EPS）组成、大小多样性、摩尔质量和大小分布的影响。比较了离心、EDTA 和甲醛/NaOH 提取的 EPS。结果表明，提取方法影响了两种菌株 EPS 中的 TOC、TN 和总蛋白含量。然而，EDTA 和甲醛/NaOH 方法在胞外多糖成分上没有差异。不同方法得到的 EPS 具有相似的官能团和荧光模式，但相对丰度取决于方法。提取方法还影响了摩尔质量和大小分布、HP SEC 多样性以及不同处理和细菌菌株之间的差异。
