Cawthron Institute, Nelson 7010, New Zealand.
University of Waikato Coastal Marine Field Station, Sulphur Point, Tauranga 3110, New Zealand.
Toxins (Basel). 2019 Jul 24;11(8):435. doi: 10.3390/toxins11080435.
Understanding of colony specific properties of cyanobacteria in the natural environment has been challenging because sampling methods disaggregate colonies and there are often delays before they can be isolated and preserved. is a ubiquitous cyanobacteria that forms large colonies in situ and often produces microcystins, a potent hepatotoxin. In the present study a new cryo-sampling technique was used to collect intact colonies in situ by embedding them in a sheet of ice. Thirty-two of these colonies were investigated with image analysis, liquid chromatography-mass spectrometry, quantitative polymerase chain reaction and high-throughput sequencing to assess their volume, microcystin quota and internal transcribed spacer (ITS) genotype diversity. Microcystin quotas were positively correlated to colony volume ( = 0.32; = 0.004). Individual colonies had low ITS genotype diversity and one ITS operational taxonomic unit predominated in all samples. This study demonstrates the utility of the cryo-sampling method to enhance the understanding of colony-specific properties of cyanobacteria with higher precision than previously possible.
由于采样方法会使蓝藻聚集体解体，而且在对其进行分离和保存之前往往需要等待一段时间，因此，对自然环境中蓝藻聚集体特定属性的了解一直具有挑战性。鱼腥藻是一种普遍存在的蓝藻，它在原位形成大的聚集体，并且常常产生微囊藻毒素，这是一种强效的肝毒素。在本研究中，采用了一种新的冷冻采样技术，通过将其嵌入冰片中，原位收集完整的鱼腥藻聚集体。对其中的 32 个鱼腥藻聚集体进行了图像分析、液相色谱-质谱分析、定量聚合酶链反应和高通量测序，以评估其体积、微囊藻毒素配额和内部转录间隔区（ITS）基因型多样性。微囊藻毒素配额与聚集体体积呈正相关（ = 0.32； = 0.004）。单个聚集体的 ITS 基因型多样性较低，所有样本中均以一种 ITS 操作分类单元为主导。这项研究表明，冷冻采样方法可用于增强对蓝藻聚集体特定属性的理解，其精度高于以往可能达到的水平。
