Center for Inflammation, Immunity & Infection, and Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA.
Mol Ther. 2012 Jul;20(7):1472-80. doi: 10.1038/mt.2012.69. Epub 2012 Apr 17.
In this study, we tested the hypothesis that DNA vaccination in the skin using microneedles improves protective immunity compared to conventional intramuscular (i.m.) injection of a plasmid DNA vaccine encoding the influenza hemagglutinin (HA). In vivo fluorescence imaging demonstrated the expression of a reporter gene delivered to the skin using a solid microneedle patch coated with plasmid DNA. Vaccination at a low dose (3 µg HA DNA) using microneedles generated significantly stronger humoral immune responses and better protective responses post-challenge compared to i.m. vaccination at either low or high (10 µg HA DNA) dose. Vaccination using microneedles at a high (10 µg) dose further generated improved post-challenge protection, as measured by survival, recall antibody-secreting cell responses in spleen and bone marrow, and interferon (IFN)-γ cytokine T-cell responses. This study demonstrates that DNA vaccination in the skin using microneedles induces higher humoral and cellular immune responses as well as improves protective immunity compared to conventional i.m. injection of HA DNA vaccine.
在这项研究中，我们测试了一个假设，即使用微针在皮肤中进行 DNA 疫苗接种可以改善针对流感血凝素 (HA) 的质粒 DNA 疫苗的保护免疫，与传统的肌肉内 (i.m.) 注射相比。体内荧光成像显示，使用涂有质粒 DNA 的固体微针贴片将报告基因递送到皮肤中。与低剂量 (3 µg HA DNA) 的 i.m. 疫苗接种相比，微针接种产生了更强的体液免疫反应和更好的保护反应。高剂量（10 µg HA DNA）的微针接种进一步提高了保护效果，如通过存活率、脾脏和骨髓中回忆抗体分泌细胞反应以及干扰素 (IFN)-γ 细胞因子 T 细胞反应来衡量。这项研究表明，与传统的 i.m. 注射 HA DNA 疫苗相比，使用微针在皮肤中进行 DNA 疫苗接种可诱导更高的体液和细胞免疫反应，并改善保护免疫。
