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单次接种病毒样颗粒对 H5N1 流感病毒的保护性免疫。

Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles.

机构信息

Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, Atlanta, Georgia, USA.

出版信息

Virology. 2010 Sep 15;405(1):165-75. doi: 10.1016/j.virol.2010.05.034. Epub 2010 Jun 26.

DOI:10.1016/j.virol.2010.05.034
PMID:20580392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2925114/
Abstract

We generated influenza virus-like particles (VLPs) containing the wild type (WT) H5 hemagglutinin (HA) from A/Viet Nam/1203/04 virus or a mutant H5 HA with a deletion of the multibasic cleavage motif. VLPs containing mutant H5 HA were found to be as immunogenic as VLPs containing WT HA. A single intramuscular vaccination with either type of H5 VLPs provided complete protection against lethal challenge. In contrast, the recombinant H5 HA vaccine was less immunogenic and vaccination even with a 5 fold higher dose did not induce protective immunity. VLP vaccines were superior to the recombinant HA in inducing T helper type 1 immune responses, hemagglutination inhibition titers, and antibody secreting cells, which significantly contribute to inducing protective immunity after a single dose vaccination. This study provides insights into the potential mechanisms of improved immunogenicity by H5 VLP vaccines as an approach to improve the protective efficacy against potential pandemic viruses.

摘要

我们从 A/Viet Nam/1203/04 病毒或缺失多碱性裂解基序的突变型 H5 HA 中生成了含有野生型 (WT) H5 血凝素 (HA) 的流感病毒样颗粒 (VLPs)。含有突变型 H5 HA 的 VLPs 被发现与含有 WT HA 的 VLPs 具有同等的免疫原性。单次肌肉内接种任何一种 H5 VLPs 均可提供针对致死性挑战的完全保护。相比之下，重组 H5 HA 疫苗的免疫原性较低，即使接种 5 倍更高剂量也不能诱导保护性免疫。VLP 疫苗在诱导 T 辅助细胞 1 型免疫反应、血凝抑制滴度和抗体分泌细胞方面优于重组 HA，这对单次接种后诱导保护性免疫有重要贡献。本研究深入了解了 H5 VLP 疫苗提高免疫原性的潜在机制，作为提高针对潜在大流行病毒的保护效力的一种方法。

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2
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Euro Surveill. 2009 May 28;14(21):19224. doi: 10.2807/ese.14.21.19224-en.
3
Induction of long-term protective immune responses by influenza H5N1 virus-like particles.流感H5N1病毒样颗粒诱导长期保护性免疫反应。
病毒样颗粒中的神经氨酸酶有助于抵御高剂量禽流感病毒攻毒感染。
Pathogens. 2021 Oct 7;10(10):1291. doi: 10.3390/pathogens10101291.
4
Evaluation of Protective Efficacy of Influenza Virus Like Particles Prepared from H5N1 Virus of Clade 2.2.1.2 in Chickens.对由2.2.1.2分支H5N1病毒制备的流感病毒样颗粒在鸡体内的保护效果评估。
Vaccines (Basel). 2021 Jul 1;9(7):715. doi: 10.3390/vaccines9070715.
5
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Sci Rep. 2021 Jul 2;11(1):13786. doi: 10.1038/s41598-021-93060-8.
6
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7
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PLoS One. 2019 Jun 27;14(6):e0216871. doi: 10.1371/journal.pone.0216871. eCollection 2019.
PLoS One. 2009;4(3):e4667. doi: 10.1371/journal.pone.0004667. Epub 2009 Mar 2.
4
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Nat Struct Mol Biol. 2009 Mar;16(3):265-73. doi: 10.1038/nsmb.1566. Epub 2009 Feb 22.
5
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Vaccine. 2009 Jan 22;27(4):530-41. doi: 10.1016/j.vaccine.2008.11.011. Epub 2008 Nov 19.
6
Evolution of highly pathogenic H5N1 avian influenza viruses in Vietnam between 2001 and 2007.2001年至2007年间越南高致病性H5N1禽流感病毒的演变
PLoS One. 2008;3(10):e3462. doi: 10.1371/journal.pone.0003462. Epub 2008 Oct 21.
7
Cross-clade protective immune responses to influenza viruses with H5N1 HA and NA elicited by an influenza virus-like particle.流感病毒样颗粒引发的针对具有H5N1血凝素和神经氨酸酶的流感病毒的跨谱系保护性免疫反应。
PLoS One. 2008 Jan 30;3(1):e1501. doi: 10.1371/journal.pone.0001501.
8
Scientific barriers to developing vaccines against avian influenza viruses.开发抗禽流感病毒疫苗的科学障碍。
Nat Rev Immunol. 2007 Apr;7(4):267-78. doi: 10.1038/nri2054.
9
Influenza virus-like particles elicit broader immune responses than whole virion inactivated influenza virus or recombinant hemagglutinin.流感病毒样颗粒比全病毒灭活流感病毒或重组血凝素引发更广泛的免疫反应。
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10
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J Virol. 2007 Apr;81(7):3514-24. doi: 10.1128/JVI.02052-06. Epub 2007 Jan 24.