Division of Gastroenterology and Hepatology, Department of Internal Medicine, Maastricht University Medical Center, Maastricht, The Netherlands.
Inflamm Bowel Dis. 2013 Jan;19(1):124-31. doi: 10.1002/ibd.22976.
While the role of bacteria as an etiological factor triggering relapse in inflammatory bowel disease (IBD) has been studied extensively, little is known of the role of enteric viruses. We aimed to prospectively study the prevalence and risk factors for common enteropathogenic viruses in IBD patients in relation to disease activity.
IBD patients visiting the outpatient clinic of the Maastricht University Medical Center were included in a prospective cohort with a follow-up of 1 year. Every 3 months and during relapses, fecal samples, demographic, and clinical data were collected and disease activity was scored. A fecal sample from patients at baseline (Crohn's disease [CD] n = 170, ulcerative colitis [UC] n = 116) and an additional sample from a subgroup with changing disease activity during follow-up (CD n = 57, UC n = 31) were analyzed for the presence of rotavirus, norovirus GI and GII, human astrovirus, and adenovirus using quantitative polymerase chain reaction (qPCR).
Overall viral pathogen detection, defined by the detection of at least one of the studied viruses, at baseline was 5.2% and differed neither between CD (6.5%) or UC patients (3.4%) (P = 0.20), nor between active disease (4.7%) and remission (5.5%) (P = 0.79). Within the subgroup of patients with changing disease activity no association was found between overall viral pathogen detection and disease activity (P = 0.39). Using multivariate logistic regression, age, gender, disease subtype, disease activity, medication, and season of sampling were not associated with overall viral pathogen detection.
Enteropathogenic viruses are not frequently observed in a consecutive cohort of IBD patients and are not a common trigger for active disease in daily clinical practice.
虽然细菌作为炎症性肠病（IBD）复发的病因因素的作用已被广泛研究，但对于肠病毒的作用知之甚少。我们旨在前瞻性研究 IBD 患者中常见肠致病性病毒的患病率和危险因素与疾病活动度的关系。
我们纳入了在马斯特里赫特大学医学中心门诊就诊的 IBD 患者进行前瞻性队列研究，随访时间为 1 年。每 3 个月和在复发期间，收集粪便样本、人口统计学和临床数据，并对疾病活动度进行评分。对基线时的患者粪便样本（克罗恩病 [CD] n = 170，溃疡性结肠炎 [UC] n = 116）和随访期间疾病活动度变化的亚组中的额外样本（CD n = 57，UC n = 31）进行轮状病毒、诺如病毒 GI 和 GII、人星状病毒和腺病毒的检测，使用定量聚合酶链反应（qPCR）。
总体病毒病原体检测，定义为至少检测到一种研究病毒，基线时为 5.2%，在 CD（6.5%）或 UC 患者（3.4%）之间无差异（P = 0.20），也与活动期疾病（4.7%）和缓解期（5.5%）之间无差异（P = 0.79）。在疾病活动度变化的亚组中，总体病毒病原体检测与疾病活动度之间无关联（P = 0.39）。使用多变量逻辑回归，年龄、性别、疾病亚型、疾病活动度、药物和采样季节与总体病毒病原体检测无关。
肠致病性病毒在连续的 IBD 患者队列中并不常见，在日常临床实践中也不是活动期疾病的常见诱因。
