Yu Shaojuan, Wang Fuli, Zhang Kui, Zhang Yun, Yang Kun, Cheng Manli, Song Chaojun, Jin Boquan
Department of Cardiology, First Hospital of Xi'an, Xi'an, PR China.
Hybridoma (Larchmt). 2012 Apr;31(2):142-5. doi: 10.1089/hyb.2011.0103.
PEX14 is an integral membrane protein essential for protein docking onto the peroxisomes and is a bi-functional protein capable of acting as a transcriptional co-repressor and a polypeptide transport modulator. Further studies showed that PEX14 is the sole peroxin that has a unique dual function in peroxisome formation and selective degradation. Its RNA transcripts are ubiquitously expressed; there is, however, no data on the expression profiles of PEX14 at the protein level due to a lack of MAbs suitable for immunohistochemical staining, thus hindering studies on its functions. In the present study, we generated one MAb that could be used in immunocytochemistry and immunohistochemistry and investigated PEX14 expression in normal and malignant human tissues. In contrast to the ubiquitous expression of its RNA transcripts, there is no PEX14 protein expression in normal human tissue, including liver, spleen, lung, rectum, brain, prostate, breast, ovary, and stomach. Protein expression of PEX14 was dramatically upregulated in some malignancies. Presented here are the first data on the expression profiles of PEX14 at the protein level, which will further our understanding of its functions.
PEX14是一种整合膜蛋白，对于蛋白质停靠到过氧化物酶体上至关重要，并且是一种双功能蛋白，能够作为转录共抑制因子和多肽转运调节剂发挥作用。进一步的研究表明，PEX14是唯一在过氧化物酶体形成和选择性降解中具有独特双重功能的过氧化物酶。其RNA转录本广泛表达；然而，由于缺乏适用于免疫组织化学染色的单克隆抗体，尚无关于PEX14蛋白水平表达谱的数据，从而阻碍了对其功能的研究。在本研究中，我们制备了一种可用于免疫细胞化学和免疫组织化学的单克隆抗体，并研究了PEX14在正常和恶性人类组织中的表达。与其RNA转录本的广泛表达相反，在包括肝脏、脾脏、肺、直肠、脑、前列腺、乳腺、卵巢和胃在内的正常人类组织中没有PEX14蛋白表达。PEX14的蛋白表达在某些恶性肿瘤中显著上调。本文首次报道了PEX14蛋白水平的表达谱数据，这将加深我们对其功能的理解。
