Gwen Knapp Center for Lupus and Immunology Research, The University of Chicago, IL 60637, USA.
Mol Immunol. 2012 Jul;51(3-4):273-82. doi: 10.1016/j.molimm.2012.03.028. Epub 2012 Apr 18.
We have developed a microarray to study the expression of L-chain V genes (V(L) genes) in healthy and SLE patient peripheral κ- and λ-sorted B cells. In all repertoires tested, one V(L) gene accounts for over 10% of all gene V(L) expression, consistent with positive selection acting on L-chains. While a few V(L) genes were highly expressed in all individuals, most V(L) genes were expressed at different levels. Some V(L) genes (5 out of a total of 78) were not detected. We attribute their absence from the repertoire to negative selection. Positive selection and negative selection were also found in SLE repertoires, but expression of V(L) genes was different; the differences point to less regulation of V(L) gene repertoires in SLE. Our data shows that V(L) gene expression is variable and supports a model where the L-chain repertoire is generated by both positive and negative selection on L-chains.
我们开发了一种微阵列来研究健康人和 SLE 患者外周κ和λ分选 B 细胞中 L 链 V 基因（V(L) 基因）的表达。在所测试的所有库中，一个 V(L) 基因占所有基因 V(L) 表达的 10%以上，这与 L 链上的正选择一致。虽然少数 V(L) 基因在所有个体中都高度表达，但大多数 V(L) 基因的表达水平不同。一些 V(L) 基因（总共 78 个中的 5 个）未被检测到。我们将它们从库中缺失归因于负选择。在 SLE 库中也发现了正选择和负选择，但 V(L) 基因的表达不同；这些差异表明 SLE 中 V(L) 基因库的调控较少。我们的数据表明 V(L) 基因表达是可变的，并支持 L 链上的正选择和负选择共同产生 L 链库的模型。
