Wang Yong-fu, Pang Chun-yan, Ma Xiu-fen, An Yan, Feng Xiu-yuan
Department of Rheumatology and Immunology, First Affiliated Hospital Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2012 Apr 18;44(2):229-32.
To investigate the expressions of GRα mRNA and GRβ mRNA in the peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients, in order to reveal the role of GR mRNA in the pathogenesis of SLE and analyze the relationship between GR mRNA and SLEDAI score, dsDNA, cardiovascular involvement.
Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to semiquantitatively analyze GRα mRNA and GRβ mRNA expressions in 104 SLE patients and 56 volunteers.
The level of GRα mRNA was lower in the SLE group (the relative level was 1.24±0.97)than in the control group (the relative level was 2.31±1.42, P<0.05), and the level of GRβ mRNA was higher in the SLE group(the relative level was 0.61±1.23) than in the control group(the relative level was 0.18±0.21, P<0.05). The level of GRα was lower in the active group (the relative level was 0.68±0.40) than in the inactive group(the relative level was 1.65±1.06, P<0.01), but the level of GRβ was higher in the active group(the relative level was 0.88±1.56) than in the inactive group(the relative level was 0.24±0.23, P<0.01); GRα mRNA was related negatively to the SLEDAI score and dsDNA, but GRβ mRNA was related positively to the SLEDAI score and dsDNA(P<0.01).The level of GRα mRNA was lower in the dsDNA positive group(the relative level was 0.89±0.66) than in the dsDNA negative group (the level was 1.54±1.10), the level of GRβ mRNA was higher in the dsDNA positive group (the relative level was 0.95±1.60) than in the dsDNA negative group (the relative level was 0.22±0.21). The level of GRβ mRNA and the value of GRβ mRNA/ GRα mRNA was obviously higher in the SLE group with cardiac involvement (the relative level was 1.02 ±1.76, the valve of GRβ / GRα was 1.10±2.02)than in the SLE group without cardiac involvement (the relative level was 0.28±0.31, the valve of GRβ / GRα was 0.32±0.32, P<0.05), and the level of GRα mRNA wasn't significant in the two groups (P>0.05).
The levels of GRα mRNA and GRβ mRNA maybe play an important role in the pathogenesis of SLE. And the levels of GRα mRNA and GRβ mRNA are related to the activity of SLE. The level of GRβ mRNA and the value of GRβ mRNA/ GRα mRNA are related with cardiovascular involvement in SLE.
研究系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMCs）中糖皮质激素受体α（GRα）mRNA和糖皮质激素受体β（GRβ）mRNA的表达，以揭示GR mRNA在SLE发病机制中的作用，并分析GR mRNA与SLE疾病活动指数（SLEDAI）评分、双链DNA（dsDNA）、心血管受累情况之间的关系。
应用逆转录-聚合酶链反应（RT-PCR）技术，对104例SLE患者和56名志愿者的GRα mRNA和GRβ mRNA表达进行半定量分析。
SLE组GRα mRNA水平（相对水平为1.24±0.97）低于对照组（相对水平为2.31±1.42，P<0.05），SLE组GRβ mRNA水平（相对水平为0.61±1.23）高于对照组（相对水平为0.18±0.21，P<0.05）。活动组GRα水平（相对水平为0.68±0.40）低于非活动组（相对水平为1.65±1.06，P<0.01），但活动组GRβ水平（相对水平为0.88±1.56）高于非活动组（相对水平为0.24±0.23，P<0.01）；GRα mRNA与SLEDAI评分和dsDNA呈负相关，而GRβ mRNA与SLEDAI评分和dsDNA呈正相关（P<0.01）。dsDNA阳性组GRα mRNA水平（相对水平为0.89±0.66）低于dsDNA阴性组（水平为1.54±1.10），dsDNA阳性组GRβ mRNA水平（相对水平为0.95±1.60）高于dsDNA阴性组（相对水平为0.22±0.21）。有心血管受累的SLE组GRβ mRNA水平及GRβ mRNA/GRα mRNA值（相对水平为1.02±1.76，GRβ/GRα值为1.10±2.02）明显高于无心血管受累的SLE组（相对水平为0.28±0.31，GRβ/GRα值为0.32±0.32，P<0.05），两组GRα mRNA水平差异无统计学意义（P>0.05）。
GRα mRNA和GRβ mRNA水平可能在SLE发病机制中起重要作用。GRα mRNA和GRβ mRNA水平与SLE的活动有关。GRβ mRNA水平及GRβ mRNA/GRα mRNA值与SLE患者心血管受累有关。
