Kou X, Li Q
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1990 Oct;30(5):397-9.
Catechol-1,2-dioxygenase (EC 1.13.11.1) catalyzes the degradation of catechol to cis, cis-muconic acid. The biochemical properties of catechol-1,2-dioxygenase from Pseudomonas putida 84103 were investigated. The optimum pH and temperature is 7.5-8.0 and 25-30 degrees C, respectively. Cu2+, Zn2+ inhibit the enzyme activity. The paper chromatograph and UV absorption spectrum of enzymatic reaction product are accordance with those of the standard muconic acid.
儿茶酚-1,2-双加氧酶（EC 1.13.11.1）催化儿茶酚降解为顺,顺-粘康酸。对来自恶臭假单胞菌84103的儿茶酚-1,2-双加氧酶的生化特性进行了研究。其最适pH和温度分别为7.5 - 8.0和25 - 30摄氏度。Cu2+、Zn2+会抑制该酶的活性。酶促反应产物的纸色谱和紫外吸收光谱与标准粘康酸的一致。
