T4D 噬菌体的琥珀突变体：它们的分离与遗传特性。

Amber mutants of bacteriophage T4D: their isolation and genetic characterization.

机构信息

Laboratory of Biophysics, University of Geneva, CH-1211 Geneva 4, Switzerland

California Institute of Technology, Pasadena, California 91125, USA

出版信息

Genetics. 2012 Mar;190(3):831-40. doi: 10.1534/genetics.112.138438.

DOI:10.1534/genetics.112.138438

PMID:22518878

Abstract

We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded that a particular subclass of base substitution mutations may give rise to amber mutants and that such mutants occur in many genes, which are widely distributed over the T4 genome.

摘要

我们分离到大量 T4D 噬菌体的突变体，这些突变体不能在大肠杆菌 B 菌株上形成噬菌斑，但能在某些其他大肠杆菌菌株，特别是 CR63 菌株上（几乎）正常生长。这些突变体被命名为琥珀（am），通过互补测试、遗传杂交和对化学诱变剂的反应进行了特征描述。结论是，碱基取代突变的一个特定亚类可能导致琥珀突变体的产生，而且这种突变体存在于许多基因中，这些基因广泛分布在 T4 基因组上。