Husby S, Høst A, Teisner B, Svehag S E
Department of Pediatrics, Odense University Hospital, Denmark.
Allergy. 1990 Oct;45(7):547-51. doi: 10.1111/j.1398-9995.1990.tb00530.x.
Seventeen children with challenge-verified cow milk allergy/intolerance (CMAI), age 3-78 months, median 12 months, were re-challenged with cow milk in increasing doses. All subjects developed symptoms, such as bronchospasm, rhinitis, diarrhoea, erythema or eczema. Blood samples were taken before and up to 24 h after the start of the challenge. The cow milk protein beta-lactoglobulin (BLG) was determined in serum with ELISA (lower detection limit 0.3 micrograms/l). BLG was detectable in five children at low levels (below 2 micrograms/l). Analysis of the size distribution of the BLG by size exclusion chromatography indicated immunoreactive material as small aggregates. Plasma samples were analysed by electroimmunoassay for complement factor split product C3d, which was not demonstrable above background values in any of the cases. CMAI in infants and children may not be related to systemic activation of the complement system and may be elicited without considerable amounts of immunoreactive BLG in the circulation.
17名经激发试验确诊为牛奶过敏/不耐受（CMAI）的儿童，年龄3至78个月，中位数为12个月，接受了递增剂量的牛奶激发试验。所有受试者均出现症状，如支气管痉挛、鼻炎、腹泻、红斑或湿疹。在激发试验开始前及开始后长达24小时采集血样。用酶联免疫吸附测定法（ELISA）（检测下限0.3微克/升）测定血清中的牛奶蛋白β-乳球蛋白（BLG）。5名儿童血清中可检测到低水平（低于2微克/升）的BLG。通过尺寸排阻色谱法分析BLG的大小分布，结果表明免疫反应性物质为小聚集体。用电免疫测定法分析血浆样品中的补体因子裂解产物C3d，在任何病例中均未发现其高于背景值。婴幼儿的CMAI可能与补体系统的全身激活无关，且在循环中没有相当数量的免疫反应性BLG的情况下也可能引发。
