Clinical Engineering, UKCTE, The Institute of Ageing and Chronic Disease, The University of Liverpool, Ground Floor, Daulby Street, Liverpool, L69 3GA, United Kingdom.
Colloids Surf B Biointerfaces. 2012 Aug 1;96:62-8. doi: 10.1016/j.colsurfb.2012.03.019. Epub 2012 Apr 6.
Surfaces of polyethylene terephthalate (PET) and polypropylene (PP) have been modified by oxygen plasma. The surface hydrophilicity and changes in topography during up to 90 days storage in water and in dry air in a desiccator were analysed by dynamic contact angle test and atomic force microscopy (AFM). Clear ageing effects on the plasma treated surface were observed as increases in contact angle and changes in roughness as functions of increasing storage time. However, the effect of oxygen plasma treatment to increase the hydrophilicity of surface was still evident on the treated surfaces even after 90 days storage either in dry air or in water. In protein adsorption experiments, human serum albumin (HSA) and fibrinogen (Fg) were adsorbed on untreated and oxygen plasma treated PET and PP surfaces. The quantified ATR-FTIR results showed that both HSA and Fg adsorption on PET and PP surfaces decreased after oxygen plasma treatment, with the effect most evident for HSA. Although for both proteins adsorption increased with ageing, the amount of adsorbed proteins was still lower than untreated surface at 30 days. This suggests the shelf life of oxygen plasma treated samples could be as long as 30 days.
聚对苯二甲酸乙二醇酯（PET）和聚丙烯（PP）的表面已通过氧等离子体进行改性。通过动态接触角测试和原子力显微镜（AFM）分析了长达 90 天在水中和干燥器中的干燥空气中储存期间表面亲水性和形貌变化。观察到等离子体处理表面的明显老化效应，表现为接触角随储存时间的增加而增加以及粗糙度的变化。然而，即使在 90 天的干燥空气或水中储存后，氧等离子体处理仍能显著提高表面的亲水性。在蛋白质吸附实验中，人血清白蛋白（HSA）和纤维蛋白原（Fg）被吸附在未经处理和氧等离子体处理的 PET 和 PP 表面上。定量 ATR-FTIR 结果表明，氧等离子体处理后 HSA 和 Fg 在 PET 和 PP 表面上的吸附均减少，对 HSA 的影响最明显。尽管两种蛋白质的吸附随时间的延长而增加，但在 30 天时，吸附的蛋白质量仍低于未经处理的表面。这表明氧等离子体处理样品的保质期可能长达 30 天。
