Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, Columbia, SC 29208, USA.
Acta Biomater. 2012 Aug;8(8):2978-85. doi: 10.1016/j.actbio.2012.04.027. Epub 2012 Apr 20.
Turnip yellow mosaic virus (TYMV) is a stable 28 nm icosahedral plant virus that can be isolated in gram quantities. In order to study the polyvalent effect of Arg-Gly-Asp (RGD) clustering on the response of bone marrow stem cells (BMSCs), an RGD motif was genetically displayed on the coat protein of the TYMV capsid. Composite films composed of either wild-type TYMV or TYMV-RGD44, in combination with poly(allylamine hydrochloride) (PAH), were fabricated by a layer-by-layer adsorption of virus and PAH. The deposition process was studied by quartz crystal microbalance, UV-visible spectroscopy and atomic force microscopy. BMSC adhesion assays showed enhanced cell adhesion and spreading on TYMV-RGD44 coated substrates compared to native TYMV. These results demonstrate the potential of TYMV as a viable scaffold for bioactive peptide display and cell culturing studies.
芜菁黄花叶病毒（TYMV）是一种稳定的 28nm 二十面体植物病毒，能够以克为单位进行分离。为了研究 Arg-Gly-Asp（RGD）聚类对骨髓基质细胞（BMSCs）反应的多效性，在 TYMV 衣壳蛋白上遗传展示了一个 RGD 基序。通过逐层吸附病毒和 PAH，制备了由野生型 TYMV 或 TYMV-RGD44 与聚（烯丙基胺盐酸盐）（PAH）组成的复合膜。通过石英晶体微天平、紫外-可见分光光度法和原子力显微镜研究了沉积过程。BMSC 黏附实验表明，与天然 TYMV 相比，RGD44 修饰的 TYMV 涂层基底上细胞黏附和铺展得到增强。这些结果表明，TYMV 作为生物活性肽展示和细胞培养研究的可行支架具有潜力。
