Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Bioresour Technol. 2012 Jul;116:290-4. doi: 10.1016/j.biortech.2012.03.068. Epub 2012 Mar 29.
The filamentous, heterocystous, diazotrophic cyanobacterium, Anabaena torulosa was found to bind uranium from aqueous solutions containing 100 μM uranyl carbonate at pH 7.8. The uranyl sequestration kinetics exhibited (a) an initial rapid phase, binding 48% uranium within 30 min resulting in a loading of 56 mg U g(-1) of dry wt, followed by (b) a slower phase, binding 65% uranium with resultant loading of 77.35 mg U g(-1) in 24h. Energy Dispersive X-ray fluorescence spectroscopy of uranium loaded biomass revealed all components of UL X-rays (UL(l), UL(α), UL(β1) and UL(β2)). Heat killed cells or extracellular polysaccharides derived from live cells exhibited limited uranyl binding (~26%) highlighting the importance of cell viability for optimum uranyl binding. The present study revealed the involvement of acid soluble polyphosphates in uranium accumulation by this brackish water cyanobacterium.
发现丝状异形胞固氮蓝藻鱼腥藻能从 pH 值为 7.8 的含 100μM 碳酸铀酰的水溶液中吸附铀。铀的螯合动力学表现出（a）初始快速相，在 30 分钟内结合 48%的铀，导致 56mgU/g（-1）的干重负载，随后（b）较慢的相，结合 65%的铀，在 24 小时内导致 77.35mgU/g（-1）的负载。铀负载生物量的能量色散 X 射线荧光光谱显示了 UL X 射线（UL(l)、UL(α)、UL(β1)和 UL(β2)）的所有成分。热杀死的细胞或来自活细胞的细胞外多糖对铀酰的结合能力有限（约 26%），这突出了细胞活力对最佳铀酰结合的重要性。本研究揭示了酸性可溶性多磷酸盐在这种咸水蓝藻积累铀中的作用。
