Department of Chemistry and Biochemistry, Seton Hall University, 400 South Orange Ave., S. Orange, NJ 07079, USA.
J Chromatogr A. 2012 Jun 1;1240:104-12. doi: 10.1016/j.chroma.2012.03.087. Epub 2012 Apr 3.
Excess adsorption isotherms of acetonitrile and methanol from water were measured on eight commercial columns. Columns used in this study represent latest examples in column development and include three different poroshell columns (Kinetex-C18, Acsentis-C18 and Halo-C18) as well as conventional columns with significantly different adsorbent geometry (Allure-C18, YMC-C18) and various hybrid-silica columns (Gemini-C18, Xterra-C18 and XBridge-C18). Comparison of the excess adsorption isotherms measured on all these columns and expressed in surface specific form demonstrated significant similarity of the adsorption properties of all columns, which allows us to introduce the "standard adsorption isotherm" for reversed-phase C18-type columns. The methodology of the evaluation of the total amount of adsorbent in the column and effective surface area of the C18 modified adsorbent is also discussed. These terms are critical for successful evaluation of surface specific parameters.
在八种商业柱上测量了乙腈和甲醇从水中的过剩吸附等温线。本研究中使用的柱代表了柱发展的最新范例，包括三种不同的 Poroshell 柱（Kinetex-C18、Acentis-C18 和 Halo-C18）以及具有明显不同的吸附剂几何形状的传统柱（Allure-C18、YMC-C18）和各种混合硅胶柱（Gemini-C18、Xterra-C18 和 XBridge-C18）。对所有这些柱上测量的过剩吸附等温线进行比较，并以表面特定形式表示，证明了所有柱的吸附性能具有显著的相似性，这使我们能够为反相 C18 型柱引入“标准吸附等温线”。还讨论了评估柱中吸附剂总量和 C18 改性吸附剂有效表面积的方法。这些术语对于成功评估表面特定参数至关重要。
