Olafsson Ingvar H, Vilhjalmsson Dadi Th, Thormodsson Finnbogi R
Faculty of Medicine, Department of Neurosurgery, University of Iceland and Landspitali University Hospital, Reykjavik, Iceland.
Methods Mol Biol. 2012;849:245-59. doi: 10.1007/978-1-61779-551-0_17.
Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer's disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs. First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVECs), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described.
脑淀粉样血管病（CAA）是由淀粉样蛋白在大脑皮质和软脑膜的动脉中积聚所致。这种病症与年龄相关，在阿尔茨海默病（AD）中尤为普遍，也是某些遗传性疾病（即冰岛遗传性脑出血伴淀粉样变性，HCHWA-I）的主要特征。血管平滑肌细胞（VSMC）似乎在CAA的发展中起着至关重要的作用，这使得它们非常适合作为研究该疾病和筛选可能治疗方法的实验模型。我们描述了两种分离和培养人VSMC的不同方法。第一种方法是使用人脐带作为获取大量强健细胞的简便来源，第二种方法是使用脑组织，脑组织可提供合适的脑VSMC，但操作起来更具难度。脐带还能提供人脐静脉内皮细胞（HUVEC），这是血管研究中有用的原代细胞。最后，描述了分离出的血管细胞的培养、保存和鉴定方法。
