Schmidt Stephen D, Nixon Ralph A, Mathews Paul M
Center for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY, USA.
Methods Mol Biol. 2012;849:493-506. doi: 10.1007/978-1-61779-551-0_33.
Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate multiple characterizations can be done, including western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Aβ in the brain of a transgenic mouse model of β-amyloid deposition. The ELISA technique is described in detail in Chapter 34.
含有淀粉样蛋白的组织，无论是来自人类患者还是疾病的动物模型，通常都通过各种生化和免疫组化技术进行表征，其中许多技术在本卷中有详细描述。在本章中，我们描述了一种在这些分析之前对组织进行匀浆的简单技术。该技术特别适合于对单个小鼠脑半球进行大量不同的生化分析。从这种匀浆开始，可以进行多种表征，包括蛋白质印迹分析和膜相关蛋白的分离，此处均有描述。还可以对组织匀浆轻松进行其他分析，包括对β-淀粉样蛋白沉积转基因小鼠模型大脑中Aβ的ELISA定量分析。ELISA技术在第34章中有详细描述。
