Neumann Gabriele, Ozawa Makoto, Kawaoka Yoshihiro
Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Methods Mol Biol. 2012;865:193-206. doi: 10.1007/978-1-61779-621-0_12.
The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility. Moreover, this technology is now being used to generate approved influenza virus vaccines and develop novel vaccines to combat seasonal and (future) pandemic influenza viruses. Several variations of the original system have been established, all of which are considerably robust and efficient.
随意改造流感病毒的能力彻底改变了流感研究。反向遗传学已被用于产生突变或重配流感病毒，以评估它们的复制、毒力、致病性、宿主范围和传播性。此外，这项技术目前正被用于生产已获批准的流感病毒疫苗，并研发新型疫苗以对抗季节性流感病毒和（未来的）大流行性流感病毒。原始系统的几种变体已经建立，所有这些变体都相当强大且高效。
