Meng Fei, Zhao Xiaoyun, Zhang Shulan
Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, 110004, China.
Department of Microbiology and Cell Biology, Shenyang Pharmaceutical University School of Life Science and Biopharmaceutics, Shenyang, 110016, China.
J Huazhong Univ Sci Technolog Med Sci. 2012 Apr;32(2):247-251. doi: 10.1007/s11596-012-0044-4. Epub 2012 Apr 20.
This study investigated the expression and prognostic value of SHP-2 in cervical cancer caused by human papillomavirus (HPV) infection. Forty-five specimens from patients with cervical cancer (stage I-III), 32 specimens from patients with cervical intraepithelial neoplasia (CIN) (I, II) and 20 normal cervical samples from patients with hysteromyoma were collected in Department of Pathology for comparison. The expression levels of SHP-2 and IFN-β proteins were detected by using immunohistochemistry. The mRNA expression level of SHP-2 was detected by using quantitative real-time polymerase chain reaction (PCR). HPVs were detected by HPV GenoArray Test. The Spearman correlation was used to compare the expression level of SHP-2 in HPV infected cervical cancer vs non-HPV infected normal cervix. The level of SHP-2 protein expression in the cancer tissues (88.8%) was significantly higher than in CIN tissues (62.5%) and normal cervixes (45%) (P<0.05 and P<0.05, respectively). The SHP-2 mRNA levels in the cancer tissues were upregulated as compared with those in the normal cervixes (P<0.05). Twenty-one (46.7%) cervical cancers, 25 (78.1%) CINs and 17 (85%) normal cervixes showed IFN-β positive staining in cytoplasm. There was statistically significant difference in the expression rate of IFN-β between cervical cancer and normal cervix (χ (2)=8.378, P<0.05) as well as between cervical cancer and CIN (χ (2)=7.695, P<0.05). HPV16/18 infections could be found in normal cervixs (15%), CINs (68.7%) and cervical cancers (84.4%). There was a correlation between HPV infection and SHP-2 expression in cervical cancer (r (s)=0.653, P<0.05). SHP-2 may be a useful prognostic and diagnostic indicator for HPV infected cervical cancer. In cervical cancers, SHP-2 mRNA and protein overexpression was associated with IFN-β lower-expression.
本研究调查了含SHP-2在人乳头瘤病毒（HPV）感染所致宫颈癌中的表达及预后价值。从病理学系收集了45例宫颈癌患者（I-III期）的标本、32例宫颈上皮内瘤变（CIN）（I、II级）患者的标本以及20例子宫肌瘤患者的正常宫颈样本用于比较。采用免疫组织化学法检测SHP-2和IFN-β蛋白的表达水平。采用定量实时聚合酶链反应（PCR）检测SHP-2的mRNA表达水平。通过HPV基因芯片检测法检测HPV。采用Spearman相关性分析比较HPV感染的宫颈癌与未感染HPV的正常宫颈中SHP-2的表达水平。癌组织中SHP-2蛋白表达水平（88.8%）显著高于CIN组织（62.5%）和正常宫颈组织（45%）（P分别<0.05和<0.05）。与正常宫颈组织相比，癌组织中SHP-2的mRNA水平上调（P<0.05）。21例（46.7%）宫颈癌、25例（78.1%）CIN和17例（85%）正常宫颈的细胞质中出现IFN-β阳性染色。宫颈癌与正常宫颈之间以及宫颈癌与CIN之间IFN-β的表达率存在统计学显著差异（χ(2)=8.378，P<0.05；χ(2)=7.695，P<0.05）。在正常宫颈（15%）、CIN（68.7%）和宫颈癌（84.4%）中均可检测到HPV16/18感染。宫颈癌中HPV感染与SHP-2表达之间存在相关性（r(s)=0.653，P<0.05）。SHP-2可能是HPV感染宫颈癌的有用预后和诊断指标。在宫颈癌中，SHP-2的mRNA和蛋白过表达与IFN-β低表达相关。
