Subbarao K V, Hertz L
Department of Pharmacology, University of Saskatchewan, Saskatoon, Canada.
Brain Res. 1990 Sep 17;527(2):346-9. doi: 10.1016/0006-8993(90)91157-c.
Noradrenaline effects on oxidative metabolism (tricarboxylic acid cycle activity) were examined by measuring the formation of 14CO2 from labeled aspartate in primary cultures of cerebral astrocytes and neurons and of cerebellar granule cells. At all time periods studied, CO2 formation in astrocytes was significantly increased in the presence of noradrenaline. The EC50 value was calculated to be 5 x 10(-7) M. No corresponding stimulation of CO2 production in cerebellar or cortical neurons was observed, suggesting that the stimulation of energy metabolism by noradrenaline in vivo may be confined to astrocytes.
通过测量原代培养的脑星形胶质细胞、神经元以及小脑颗粒细胞中标记天冬氨酸生成(^{14}CO_2)的情况，研究了去甲肾上腺素对氧化代谢（三羧酸循环活性）的影响。在所有研究的时间段内，去甲肾上腺素存在时星形胶质细胞中的(CO_2)生成显著增加。计算得出的半数有效浓度（EC50）值为(5×10^{-7})M。未观察到去甲肾上腺素对小脑或皮质神经元的(CO_2)生成有相应刺激作用，这表明体内去甲肾上腺素对能量代谢的刺激作用可能局限于星形胶质细胞。
