• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

线粒体膜系统的组装。酵母细胞色素氧化酶亚基2的DNA序列。

Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase.

作者信息

Coruzzi G, Tzagoloff A

出版信息

J Biol Chem. 1979 Sep 25;254(18):9324-30.

PMID:225327
Abstract

A cytoplasmic "petitie" mutant of Saccharomyces cerevisiae (DS200/A1) has been isolated and determined to contain mitochondrial genetic markers in the oxi 1 locus. This locus has previously been reported to code for the structural gene of subunit 2 of cytochrome oxidase (Cabral et al. (1978) J. Biol. Chem. 243, 297-304). The segment of mitochondrial DNA retained in DS200/A1 has a repeat length of approximately 4500 base pairs and based on DNA sequencing contains a 756-nucleotide-long sequence that has been identified as the structural gene of subunit 2 of cytochrome oxidase. The presumptive gene sequence generates an amino acid sequence consistent with the reported molecular weight and composition of subunit 2 of yeast cytochrome oxidase. The correctness of the deduced amino acid sequence is further supported by its extensive homology to the primary structure of bovine cytochrome oxidase. The DNA segment of DS200/A1 has been located on the wild type mitochondrial DNA by comparative restriction mapping. The orientation of the COOH and NH2 termini and the direction of transcription of the gene have been determined.

摘要

已分离出酿酒酵母的一种细胞质“小菌落”突变体（DS200/A1），并确定其在oxi 1位点含有线粒体遗传标记。此前有报道称，该位点编码细胞色素氧化酶亚基2的结构基因（卡布拉尔等人，（1978年）《生物化学杂志》243卷，297 - 304页）。保留在DS200/A1中的线粒体DNA片段重复长度约为4500个碱基对，基于DNA测序，其包含一个756个核苷酸长的序列，该序列已被鉴定为细胞色素氧化酶亚基2的结构基因。推测的基因序列产生的氨基酸序列与报道的酵母细胞色素氧化酶亚基2的分子量和组成一致。推导的氨基酸序列的正确性进一步得到其与牛细胞色素氧化酶一级结构广泛同源性的支持。通过比较限制性图谱分析，已确定DS200/A1的DNA片段在野生型线粒体DNA上的位置。还确定了该基因COOH和NH2末端的方向以及转录方向。

相似文献

1
Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase.线粒体膜系统的组装。酵母细胞色素氧化酶亚基2的DNA序列。
J Biol Chem. 1979 Sep 25;254(18):9324-30.
2
Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase.线粒体膜系统的组装。酵母细胞色素氧化酶亚基1编码基因的结构与核苷酸序列。
J Biol Chem. 1980 Dec 25;255(24):11927-41.
3
Assembly of the mitochondrial membrane system. Sequence of the oxi 2 gene of yeast mitochondrial DNA.线粒体膜系统的组装。酵母线粒体DNA的oxi 2基因序列。
J Biol Chem. 1980 Jul 10;255(13):6173-80.
4
Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxi1 region of yeast mitochondrial DNA.线粒体膜系统的组装。酵母线粒体DNA氧化1区域核苷酸序列及转录本分析。
J Biol Chem. 1981 Dec 25;256(24):12780-7.
5
COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini.COX8，酵母细胞色素c氧化酶亚基VIII的结构基因。DNA序列和基因破坏表明，亚基VIII是细胞呼吸达到最高水平所必需的，并且它来源于一个在其NH2和COOH末端都有延伸的前体。
J Biol Chem. 1986 Dec 25;261(36):17192-7.
6
Isolation and characterization of COX12, the nuclear gene for a previously unrecognized subunit of Saccharomyces cerevisiae cytochrome c oxidase.酿酒酵母细胞色素c氧化酶一个先前未被识别的亚基的核基因COX12的分离与鉴定。
J Biol Chem. 1992 Nov 5;267(31):22473-80.
7
Assembly of the mitochondrial membrane system. Characterization of the oxi2 transcript and localization of its promoter in Saccharomyces cerevisiae D273-10B.线粒体膜系统的组装。酿酒酵母D273-10B中oxi2转录本的特征及其启动子的定位。
J Biol Chem. 1983 Jan 10;258(1):610-5.
8
Cloning and characterization of PET100, a gene required for the assembly of yeast cytochrome c oxidase.酵母细胞色素c氧化酶组装所需基因PET100的克隆与特性分析
J Biol Chem. 1996 Aug 2;271(31):18499-507. doi: 10.1074/jbc.271.31.18499.
9
Assembly of the mitochondrial membrane system. The DNA sequence of a mitochondrial ATPase gene in Saccharomyces cerevisiae.线粒体膜系统的组装。酿酒酵母中线粒体ATP酶基因的DNA序列。
J Biol Chem. 1979 Jun 10;254(11):4617-23.
10
Assembly of the mitochondrial membrane system. Sequences of yeast mitochondrial tRNA genes.线粒体膜系统的组装。酵母线粒体tRNA基因序列。
J Biol Chem. 1980 Oct 10;255(19):9075-81.

引用本文的文献

1
Allotopic expression of elucidates Atco-driven co-assembly of cytochrome oxidase and ATP synthase.异位表达阐明了 Atco 驱动细胞色素氧化酶和 ATP 合酶的共组装。
Life Sci Alliance. 2023 Aug 21;6(11). doi: 10.26508/lsa.202301965. Print 2023 Nov.
2
Do mitochondria use efflux pumps to protect their ribosomes from antibiotics?线粒体是否利用外排泵来保护其核糖体免受抗生素的侵害？
Microbiology (Reading). 2023 Jan;169(1). doi: 10.1099/mic.0.001272.
3
Mitochondrial mutagenesis in Saccharomyces cerevisiae : V. Frequencies of different mit (-) mutants and loss of their mit (+) alleles in rho (-) clones.
酵母线粒体突变：V. rho(-)克隆中不同的mit(-)突变体的频率及其 mit(+)等位基因的丢失。
Curr Genet. 1981 Apr;3(1):57-63. doi: 10.1007/BF00419581.
4
Assembly of the mitochondrial membrane system. Organization of yeast mitochondrial DNA in the Oli1 region.线粒体膜系统的组装。酵母线粒体 DNA 在 Oli1 区域的组织。
Curr Genet. 1980 Oct;2(2):149-57. doi: 10.1007/BF00420627.
5
Recombinational analysis of OXI1 mutants and preliminary analysis of their translation products in S. cerevisiae.OXI1 突变体的重组分析及其在酿酒酵母中的翻译产物的初步分析。
Curr Genet. 1980 Jul;2(1):45-51. doi: 10.1007/BF00445693.
6
Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes.大鼠肝线粒体 DNA 片段分析，该片段包含细胞色素氧化酶亚基 I、II 和 III、ATP 酶亚基 6 以及几个 tRNA 基因。
Curr Genet. 1981 Nov;4(2):151-8. doi: 10.1007/BF00365694.
7
A maturase-like coding sequence downstream of the OXI2 gene of yeast mitochondrial DNA is interrupted by two GC clusters and a putative end-of-messenger signal.酵母线粒体 DNA 的 OXI2 基因下游有一个与成熟酶相似的编码序列，该序列被两个 GC 簇和一个假定的信使 RNA 终止信号所中断。
Curr Genet. 1984 May;8(4):307-17. doi: 10.1007/BF00419729.
8
Mitochondrial DNA of Chlamydomonas reinhardtii: the DNA sequence of a region showing homology with mammalian URF2.莱茵衣藻的线粒体 DNA：与哺乳动物 URF2 具有同源性的区域的 DNA 序列。
Curr Genet. 1984 Dec;9(1):75-82. doi: 10.1007/BF00396207.
9
The effect of paromomycin and [psi] on the suppression of mitochondrial mutations in Saccharomyces cerevisiae.巴龙霉素和[psi]对酿酒酵母线粒体突变抑制的影响。
Curr Genet. 1984 Dec;9(1):21-30. doi: 10.1007/BF00396200.
10
Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria : II. Specificity and extent of suppressor action.线粒体和核线粒体核糖体抑制剂可使酵母线粒体中的赭石密码子误读：II. 抑制剂作用的特异性和程度。
Curr Genet. 1984 Dec;9(1):11-9. doi: 10.1007/BF00396199.