Eijkman Institute for Molecular Biology, Jl, Diponegoro 69, Jakarta, Indonesia.
Cardiovasc Diabetol. 2012 Apr 25;11:41. doi: 10.1186/1475-2840-11-41.
Uncoupling protein 2 (UCP2) gene polymorphisms have been reported as genetic risk factors for obesity and type 2 diabetes mellitus (T2DM). We examined the association of commonly observed UCP2 G(-866)A (rs659366) and Ala55Val (C > T) (rs660339) single nucleotide polymorphisms (SNPs) with obesity, high fasting plasma glucose, and serum lipids in a Balinese population.
A total of 603 participants (278 urban and 325 rural subjects) were recruited from Bali Island, Indonesia. Fasting plasma glucose (FPG), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) were measured. Obesity was determined based on WHO classifications for adult Asians. Participants were genotyped for G(-866)A and Ala55Val polymorphisms of the UCP2 gene.
Obesity prevalence was higher in urban subjects (51%) as compared to rural subjects (23%). The genotype, minor allele (MAF), and heterozygosity frequencies were similar between urban and rural subjects for both SNPs. All genotype frequencies were in Hardy-Weinberg equilibrium. A combined analysis of genotypes and environment revealed that the urban subjects carrying the A/A genotype of the G(-866)A SNP have higher BMI than the rural subjects with the same genotype. Since the two SNPs showed strong linkage disequilibrium (D' = 0.946, r2 = 0.657), a haplotype analysis was performed. We found that the AT haplotype was associated with high BMI only when the urban environment was taken into account.
We have demonstrated the importance of environmental settings in studying the influence of the common UCP2 gene polymorphisms in the development of obesity in a Balinese population.
解偶联蛋白 2（UCP2）基因多态性已被报道为肥胖和 2 型糖尿病（T2DM）的遗传风险因素。我们研究了常见的 UCP2 G（-866）A（rs659366）和 Ala55Val（C> T）（rs660339）单核苷酸多态性（SNP）与肥胖、空腹血糖升高和血清脂质在巴厘岛人群中的相关性。
共招募了 603 名参与者（278 名城市和 325 名农村居民），来自印度尼西亚巴厘岛。测量空腹血糖（FPG）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）和总胆固醇（TC）。肥胖的确定基于世界卫生组织对亚洲成年人的分类。对 UCP2 基因的 G（-866）A 和 Ala55Val 多态性进行基因分型。
城市居民（51%）的肥胖患病率高于农村居民（23%）。两种 SNP 的城市和农村人群在基因型、次要等位基因（MAF）和杂合度频率上均相似。所有基因型频率均符合哈迪-温伯格平衡。基因型和环境的综合分析表明，携带 G（-866）A SNP 的 A/A 基因型的城市居民的 BMI 高于具有相同基因型的农村居民。由于两个 SNP 显示出很强的连锁不平衡（D'=0.946，r2=0.657），因此进行了单体型分析。我们发现，只有在考虑到城市环境时，AT 单体型才与高 BMI 相关。
我们已经证明了环境因素在研究常见 UCP2 基因多态性在巴厘岛人群肥胖发生中的影响的重要性。
