钠泵在犬气管上皮中的定位。
Localization of Na pumps in the tracheal epithelium of the dog.
作者信息
Widdicombe J H, Basbaum C B, Yee J Y
出版信息
J Cell Biol. 1979 Aug;82(2):380-90. doi: 10.1083/jcb.82.2.380.
Abstract
Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a Km of 10(-7) M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposures of tissue sheets to tracer showed that the submucosal side took up 10 X as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes.
摘要
狗气管上皮对[3H]哇巴因的结合表现出一个非特异性成分，它与哇巴因浓度呈线性关系，还有一个特异性可饱和成分，其Km为10(-7)M。对照实验表明，摄取的示踪剂既没有被困在细胞外间隙，也没有与组织胶原蛋白结合。高钾、代谢抑制、低钠和低温对可饱和摄取的抑制表明，结合是与钠钾ATP酶发生的。将组织片单侧暴露于示踪剂显示，黏膜下层摄取的示踪剂是管腔侧的10倍。放射自显影在所有条件下都将示踪剂摄取定位到细胞的基底外侧膜。
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