Trump B F, Berezesky I K
Department of Pathology, University of Maryland School of Medicine, Baltimore.
Clin Lab Med. 1990 Sep;10(3):531-47.
The regulation of ions within the cell is of critical importance in both acute and chronic toxicology. Recently, new methods have been developed for measuring such changes in living cells and correlating them with studies of structure, function, and biochemistry. A major revolution has occurred from the linkage between the computer and the light microscope, which has resulted in the use of digital imaging fluorescence microscopy and video intensification microscopy coupled with image analysis. These methods have already yielded much additional information and it is anticipated that their further application by pathologists and toxicologists will continue to uncover the important role of ion deregulation and toxic cell injury.
细胞内离子的调节在急性和慢性毒理学中都至关重要。最近，已经开发出了新的方法来测量活细胞中的此类变化，并将其与结构、功能和生物化学研究相关联。计算机与光学显微镜的连接带来了一场重大变革，这导致了数字成像荧光显微镜和视频增强显微镜与图像分析的结合使用。这些方法已经产生了许多额外的信息，预计病理学家和毒理学家对它们的进一步应用将继续揭示离子失调和毒性细胞损伤的重要作用。
