Une Yumi, Matsui Kumi, Tamukai Kenichi, Goka Koichi
Azabu University, Chuo-ku, Sagamihara, Kanagawa, Japan.
Dis Aquat Organ. 2012 Apr 26;98(3):243-7. doi: 10.3354/dao02442.
The purpose of this study was to establish a method for eradicating a chytrid fungus (Batrachochytrium dendrobatidis; Bd) from the Japanese giant salamander Andrias japonicus. The emerging agent (Bd) has a high rate of detection in this endangered amphibian species, which is designated as a special natural monument in Japan. Four Japanese giant salamanders with Bd confirmed by PCR assay were bathed in 0.01% itraconazole for 5 min d-1 over 10 successive days. PCR assays were conducted prior to treatment, on Days 5 and 10 of treatment, and on Days 7 and 14 post-treatment. By treatment Day 5, all individuals tested negative for Bd and remained negative until the end of the experiment. No side effects associated with itraconazole were observed. The present method appears to be a safe and effective approach for Bd eradication and may contribute to reducing the threat and spread of Bd among endangered amphibians. Notably, this study represents the first reported Bd eradication experiment involving Japanese giant salamanders.
本研究的目的是建立一种从日本大鲵（Andrias japonicus）中根除一种壶菌（蛙壶菌；Bd）的方法。这种新出现的病原体（Bd）在这种濒危两栖动物物种中的检出率很高，该物种在日本被指定为特别天然纪念物。对4只经PCR检测确认感染Bd的日本大鲵，连续10天每天用0.01%伊曲康唑浸泡5分钟。在治疗前、治疗的第5天和第10天以及治疗后的第7天和第14天进行PCR检测。到治疗第5天，所有检测个体的Bd检测均为阴性，直至实验结束一直保持阴性。未观察到与伊曲康唑相关的副作用。目前的方法似乎是一种安全有效的根除Bd的方法，可能有助于减少Bd在濒危两栖动物中的威胁和传播。值得注意的是，本研究是首次报道的涉及日本大鲵的Bd根除实验。
