Ishizawa H, Dave J R, Liu L I, Tabakoff B, Hoffman P L
Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892.
Eur J Pharmacol. 1990 Sep 18;189(2-3):119-27. doi: 10.1016/0922-4106(90)90015-p.
Vasopressin mRNA levels in the supraoptic and paraventricular nuclei of the hypothalamus, measured by in situ hybridization with a 35S-labeled RNA probe, were decreased by nearly 50% in C57BL/6NCR mice that had ingested an ethanol-containing diet for 7 days, and were tolerant to and physically dependent on ethanol. At 24 h after withdrawal, vasopressin mRNA levels in the supraoptic nucleus were still decreased, while levels in the paraventricular nucleus returned toward control values. Although plasma osmolality was increased in ethanol-fed mice, there was no increase in plasma vasopressin levels, possibly as a result of the effect of chronic ethanol ingestion to decrease vasopressin synthesis. In contrast, in mice that were dehydrated, but not fed ethanol, plasma osmolality, plasma vasopressin levels, and hypothalamic vasopressin mRNA all increased, as expected. The data suggest that chronic ethanol ingestion interferes with the synthesis and secretion of vasopressin, and may result in the reduced ability of an individual to respond to physiological stimuli for vasopressin secretion.
通过用35S标记的RNA探针进行原位杂交测量，摄入含乙醇饮食7天且对乙醇产生耐受和身体依赖的C57BL/6NCR小鼠下丘脑视上核和室旁核中的血管加压素mRNA水平降低了近50%。戒断24小时后，视上核中的血管加压素mRNA水平仍降低，而室旁核中的水平恢复至对照值。尽管喂食乙醇的小鼠血浆渗透压升高，但血浆血管加压素水平并未升高，这可能是慢性乙醇摄入降低血管加压素合成的结果。相比之下，在脱水但未喂食乙醇的小鼠中，血浆渗透压、血浆血管加压素水平和下丘脑血管加压素mRNA均如预期那样升高。数据表明，慢性乙醇摄入会干扰血管加压素的合成和分泌，并可能导致个体对血管加压素分泌的生理刺激反应能力降低。
