Larsson A, Hasselgren G
J Histochem Cytochem. 1979 May;27(5):982-8. doi: 10.1177/27.5.225377.
Two histochemical methods for demonstration of alkaline phosphatase activity, a lead pyrophosphate- anda naphtholphosphate technique, were compared. Since different results may be due to methodological differences as well as different enzyme activities, the enzymatic hydrolysis of the naphtholphosphate was visualized both by means of an azo-dye coupler and by lead-capturing of the liberated phosphate ion. Various potential inhibitors of alkaline phosphatase activity (diphosphonate, D-penicillamine, and sodium fluoride) were also tested. The use of diphosphonate and D-penicillamine resulted in inhibited or reduced staining, which could mainly be explained by an interference by these compounds with components in the incubation media rather than with the enzyme itself. The addition of sodium fluoride had no effect on the naphtholphosphate staining pattern irrespective of capturing method, whereas the odontoblastic pyrophosphate splitting alkaline phosphatase appeared to be sensitive to sodium fluoride, suggesting the presence of two alkaline phosphatases in odontoblasts.
对用于显示碱性磷酸酶活性的两种组织化学方法——焦磷酸铅法和萘磷酸法进行了比较。由于不同的结果可能是由于方法学差异以及不同的酶活性导致的，因此通过偶氮染料偶联剂以及对释放的磷酸根离子进行铅捕获这两种方式，来观察萘磷酸的酶促水解过程。还测试了碱性磷酸酶活性的各种潜在抑制剂（二膦酸盐、D-青霉胺和氟化钠）。使用二膦酸盐和D-青霉胺会导致染色受到抑制或减弱，这主要可以解释为这些化合物对孵育介质中的成分产生了干扰，而非对酶本身产生干扰。无论采用何种捕获方法，添加氟化钠对萘磷酸染色模式均无影响，而牙本质细胞的焦磷酸裂解碱性磷酸酶似乎对氟化钠敏感，这表明牙本质细胞中存在两种碱性磷酸酶。
