人血小板糖蛋白IX。cDNA的特性及该基因在3号染色体上的定位。
Human platelet glycoprotein IX. Characterization of cDNA and localization of the gene to chromosome 3.
作者信息
Hickey M J, Deaven L L, Roth G J
机构信息
Seattle Veterans Administration Medical Center, WA 98108.
出版信息
FEBS Lett. 1990 Nov 12;274(1-2):189-92. doi: 10.1016/0014-5793(90)81361-q.
Overlapping cDNAs encoding human platelet glycoprotein (Gp)IX were cloned from a human erythroleukemia cell lambda gt11 library. The possibly 'full-length' cDNA of 896 base pairs (bp) includes an open reading frame (528 bp), both 5' (222 bp) and 3' (146 bp) noncoding regions, and a poly(A) tail. Translation predicts a signal peptide of 16 amino acids and a mature protein of 160 amino acids that includes a 24 amino acid leucine-rich glycoprotein (LRG) segment. Southern blot analysis suggests the presence of a single copy of the Gp IX gene, and hybridization of Gp IX cDNA to sorted human chromosomes localizes the Gp IX gene to chromosome 3.
从人红白血病细胞λgt11文库中克隆出编码人血小板糖蛋白（Gp）IX的重叠互补DNA。这个可能为“全长”的896个碱基对（bp）的互补DNA包括一个开放阅读框（528 bp）、5'非编码区（222 bp）和3'非编码区（146 bp）以及一个多聚腺苷酸尾。翻译预测有一个16个氨基酸的信号肽和一个160个氨基酸的成熟蛋白，该成熟蛋白包含一个24个氨基酸的富含亮氨酸糖蛋白（LRG）区段。Southern印迹分析表明存在Gp IX基因的单拷贝，并且Gp IX互补DNA与分选的人类染色体杂交将Gp IX基因定位到3号染色体。
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