Jaeschke H, Farhood A, Smith C W
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
FASEB J. 1990 Dec;4(15):3355-9.
To determine the role of neutrophils in the pathogenesis of hepatic ischemia/reperfusion injury, livers from male Fischer rats were subjected to 45 min of no-flow ischemia followed by reperfusion for up to 24 h. Two phases of liver injury were identified, an initial phase during the first hour of reperfusion and a later progression phase with 80 +/- 3% hepatocyte necrosis and an 80-fold increase of neutrophil infiltration in the liver after 24 h. Pretreatment with a monoclonal antibody against neutrophils, which caused consistent neutropenia, protected the liver from reperfusion injury as indicated by 28 +/- 10% necrosis, and 84% reduction of hepatic neutrophil accumulation and a complete recovery of the hepatic ATP content. Our data suggest that the later progression phase of reperfusion injury after hepatic no-flow ischemia is mediated mainly by neutrophils.
为了确定中性粒细胞在肝缺血/再灌注损伤发病机制中的作用，对雄性Fischer大鼠的肝脏进行45分钟的无血流缺血，然后再灌注长达24小时。确定了肝损伤的两个阶段，再灌注第一小时的初始阶段和后期进展阶段，24小时后肝细胞坏死率达80±3%，肝脏中性粒细胞浸润增加80倍。用抗中性粒细胞单克隆抗体进行预处理可导致持续的中性粒细胞减少，保护肝脏免受再灌注损伤，表现为坏死率为28±10%，肝脏中性粒细胞积聚减少84%，肝脏ATP含量完全恢复。我们的数据表明，肝无血流缺血后再灌注损伤的后期进展阶段主要由中性粒细胞介导。
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