Center for Biomaterial Development and Berlin-Brandenburg Center for Regenerative Therapies, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Clin Hemorheol Microcirc. 2012;50(1-2):131-42. doi: 10.3233/CH-2010-1449.
The need for engineered devices to treat cardiovascular diseases is increasing due to an aging population and a changing lifestyle. Soft poly(n-butyl acrylate) (cPnBA) networks were recently described as polymer networks with adjustable mechanical properties and suggested as soft substrates for cells, which could potentially be used for cardiovascular implants. Vascular prostheses designed to be implanted in arteries should have an elasticity similar to blood vessels (elastic modulus at body temperature between 100 and 1200 kPa). Therefore, cPnBA networks with E-moduli of 250 kPa (cPnBA0250) and 1100 kPa (cPnBA1100) were developed. Recently, it was shown that both materials were non-cytotoxic for murin fibroblasts, human primary endothelial cells and human monocytes. However, before such newly developed polymers can be used in vivo, it has to be assured that the sterilized materials have a very low endotoxin load to avoid an unspecific activation of the immune system, which otherwise might cause local or systemic inflammatory responses and could lead to severe pathologies. In this study we investigated the immuno-compatibility of sterilized cPnBA0250 and cPnBA1100 with the help of an immuno-competent macrophage cell line as well as with whole human blood.
由于人口老龄化和生活方式的改变，对用于治疗心血管疾病的工程设备的需求正在增加。最近有人描述软聚（正）丁酯（cPnBA）网络是一种具有可调节机械性能的聚合物网络，并被提议作为细胞的软基底，这可能会被用于心血管植入物。设计用于植入动脉的血管假体应具有与血管相似的弹性（体温下的弹性模量在 100 到 1200kPa 之间）。因此，开发了弹性模量为 250kPa（cPnBA0250）和 1100kPa（cPnBA1100）的 cPnBA 网络。最近，研究表明这两种材料对鼠成纤维细胞、人原代内皮细胞和人单核细胞均无细胞毒性。然而，在这些新开发的聚合物可以在体内使用之前，必须确保经过消毒的材料的内毒素负载非常低，以避免免疫系统的非特异性激活，否则可能会导致局部或全身炎症反应，并可能导致严重的病理。在这项研究中，我们利用免疫功能健全的巨噬细胞系和全血研究了消毒后的 cPnBA0250 和 cPnBA1100 的免疫相容性。
