Pałka Katarzyna, Szymańska Jolanta, Kańska Marianna
Department of Chemistry, University of Warsaw, Pasteur 1 str, Warsaw 02-093, Poland.
Isotopes Environ Health Stud. 2013;49(1):3-8. doi: 10.1080/10256016.2012.683525. Epub 2012 Apr 27.
In this study, the kinetic isotope effects and solvent isotope effects in the reaction of the deamination of [(1R)-(2)H ] putrescine--catalysed by enzyme diamine oxidase (EC 1.4.3.6)--were determined using a non-competitive spectroscopic method. Putrescine, stereospecifically labelled with deuterium, was obtained by enzymatic decarboxylation of l-ornithine that was carried out in a fully deuteriated incubation medium.
在本研究中，使用非竞争性光谱法测定了[(1R)-(2)H]腐胺脱氨反应中的动力学同位素效应和溶剂同位素效应，该反应由二胺氧化酶（EC 1.4.3.6）催化。通过在完全氘化的孵育培养基中对L-鸟氨酸进行酶促脱羧反应，获得了立体特异性标记有氘的腐胺。
