由二胺氧化酶催化的腐胺氧化反应中的动力学和溶剂氘同位素效应。

Kinetic and solvent deuterium isotope effects in the oxidation of putrescine catalysed by enzyme diamine oxidase.

作者信息

Pałka Katarzyna, Szymańska Jolanta, Kańska Marianna

机构信息

Department of Chemistry, University of Warsaw, Pasteur 1 str, Warsaw 02-093, Poland.

出版信息

Isotopes Environ Health Stud. 2013;49(1):3-8. doi: 10.1080/10256016.2012.683525. Epub 2012 Apr 27.

DOI:10.1080/10256016.2012.683525

PMID:22540261

Abstract

In this study, the kinetic isotope effects and solvent isotope effects in the reaction of the deamination of [(1R)-(2)H ] putrescine--catalysed by enzyme diamine oxidase (EC 1.4.3.6)--were determined using a non-competitive spectroscopic method. Putrescine, stereospecifically labelled with deuterium, was obtained by enzymatic decarboxylation of l-ornithine that was carried out in a fully deuteriated incubation medium.

摘要

在本研究中，使用非竞争性光谱法测定了[(1R)-(2)H]腐胺脱氨反应中的动力学同位素效应和溶剂同位素效应，该反应由二胺氧化酶（EC 1.4.3.6）催化。通过在完全氘化的孵育培养基中对L-鸟氨酸进行酶促脱羧反应，获得了立体特异性标记有氘的腐胺。