Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada.
Biomaterials. 2012 Jul;33(21):5297-307. doi: 10.1016/j.biomaterials.2012.04.008. Epub 2012 Apr 26.
The expression of native sonic hedgehog (Shh) was significantly increased in poly(methacrylic acid-co-methyl methacrylate) bead (MAA) treated wounds at day 4 compared to both poly(methyl methacrylate) bead (PMMA) treated and untreated wounds in diabetic db/db mice. MAA beads also increased the expression of the Shh transcription factor Gli3 at day 4. Previously, topical application of MAA beads (45 mol % methacrylic acid) improved wound closure and blood vessel density in excisional wounds in these mice, while PMMA beads did not. Gene expression within the granulation tissue of healing wounds was studied to provide insight into the mechanism of vessel formation and wound healing in the presence of MAA beads. In addition to the increased expression of Shh, MAA-treated wounds had increased expression of osteopontin (OPN), IL-1β and TNF-α, (at day 7) similar to the previously reported MAA response of macrophage-like and endothelial cells in vitro.
在糖尿病 db/db 小鼠的聚（甲基丙烯酸-co-甲基丙烯酸甲酯）珠（MAA）处理的伤口中，与聚（甲基丙烯酸甲酯）珠（PMMA）处理的和未处理的伤口相比，内源性 sonic 刺猬（Shh）的表达在第 4 天明显增加。MAA 珠还在第 4 天增加了 Shh 转录因子 Gli3 的表达。先前，MAA 珠（45mol%甲基丙烯酸）的局部应用改善了这些小鼠的切口创面的闭合和血管密度，而 PMMA 珠则没有。研究了愈合创面肉芽组织中的基因表达，以深入了解存在 MAA 珠时血管形成和创面愈合的机制。除了 Shh 的表达增加外，MAA 处理的伤口还增加了骨桥蛋白（OPN）、IL-1β 和 TNF-α 的表达（第 7 天），与先前报道的 MAA 对体外巨噬细胞样细胞和内皮细胞的反应相似。
