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哺乳动物单个平滑肌细胞的力学特性。I. 一种低成本大范围微力传感器。

Mechanical properties of mammalian single smooth muscle cells. I. A low cost large range microforce transducer.

作者信息

Glerum J J, Van Mastrigt R

机构信息

Department of Urology, Erasmus University, Rotterdam, The Netherlands.

出版信息

J Muscle Res Cell Motil. 1990 Aug;11(4):331-7. doi: 10.1007/BF01766671.

DOI:10.1007/BF01766671
PMID:2254440
Abstract

A transducer has been developed for measuring the minute forces generated during isometric contractions (1.0-10.0 microN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mV microN-1, and less than 0.1 microN and less than 2.0 microN h-1), the transducer features a very wide range (100-140 microN) with good linearity, enabling measurement of contractions as well as passive force-length characteristics within one uninterrupted measurement session. Since the transducer features an independent and interchangeable force to displacement conversion system, different force ranges can be realized by inserting force conversion systems with different compliances.

摘要

已经开发出一种传感器,用于测量来自猪膀胱和人类子宫的单个平滑肌细胞在等长收缩(1.0 - 10.0微牛顿)过程中产生的微小力。除了具有高灵敏度、分辨率和稳定性(100毫伏/微牛顿、小于0.1微牛顿和小于2.0微牛顿/小时)外,该传感器还具有非常宽的测量范围(100 - 140微牛顿)和良好的线性度,能够在一次不间断的测量过程中测量收缩以及被动的力-长度特性。由于该传感器具有独立且可互换的力-位移转换系统,通过插入具有不同柔度的力转换系统,可以实现不同的力测量范围。

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J Muscle Res Cell Motil. 1990 Aug;11(4):331-7. doi: 10.1007/BF01766671.
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引用本文的文献

1
Mechanical properties of mammalian single smooth muscle cells. III. Passive properties of pig detrusor and human a terme uterus cells.
J Muscle Res Cell Motil. 1990 Oct;11(5):453-62. doi: 10.1007/BF01739765.
2
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J Muscle Res Cell Motil. 1990 Aug;11(4):338-43. doi: 10.1007/BF01766672.

本文引用的文献

1
Length-tension relation of single smooth muscle cells isolated from the pedal retractor muscle of Mytilus edulis.从蓝贻贝足缩肌分离出的单个平滑肌细胞的长度-张力关系。
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Sensitive force transducer system for mechanical studies of single isolated vascular smooth muscle cells.
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Cross-bridge elasticity in single smooth muscle cells.单个平滑肌细胞中的横桥弹性。
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A voltage-controlled force generator for calibrating sensitive transducers.
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A low-drift transducer for small forces.
J Appl Physiol. 1972 Oct;33(4):529-31. doi: 10.1152/jappl.1972.33.4.529.
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A versatile transducer system for mechanical studies of muscle.
J Appl Physiol. 1974 Sep;37(3):459-63. doi: 10.1152/jappl.1974.37.3.459.
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An ultrasensitive transducer for measurement of isometric contractile force from single heart cells.一种用于测量单个心肌细胞等长收缩力的超灵敏换能器。
Pflugers Arch. 1986 Jul;407(1):109-15. doi: 10.1007/BF00580730.
10
Mechanical properties of mammalian single smooth muscle cells. II. Evaluation of a modified technique for attachment of cells to the measurement apparatus.
J Muscle Res Cell Motil. 1990 Aug;11(4):338-43. doi: 10.1007/BF01766672.