Department of Biotechnology, Konkuk University, Chungju 380-701, Korea.
Appl Biochem Biotechnol. 2012 May;167(2):259-69. doi: 10.1007/s12010-012-9668-x. Epub 2012 Apr 28.
Japanese encephalitis virus (JEV) is an important pathogen causing febrile syndrome, encephalitis, and death. Envelop (E) glycoprotein is the major target of inducing neutralizing antibodies and protective immunity in host. In this study, E glycoprotein of JEV was expressed in Spodoptera frugiperd 9 cells as a fusion protein containing a gX signal sequence of pseudorabies virus. This purified HcE recombinant protein was evaluated for their immunogenicity and protective efficacy in guinea pig. The survival rates of guinea pig immunized with HcE protein was significantly increased over that of JE vaccine. This result indicates helpful information for developing a subunit vaccine against JEV.
日本脑炎病毒（JEV）是一种重要的病原体，可引起发热综合征、脑炎和死亡。包膜（E）糖蛋白是诱导宿主中和抗体和保护性免疫的主要靶标。在这项研究中，JEV 的 E 糖蛋白作为含有伪狂犬病病毒 gX 信号序列的融合蛋白在 Spodoptera frugiperda 9 细胞中表达。该纯化的 HcE 重组蛋白在豚鼠中评估了其免疫原性和保护效力。用 HcE 蛋白免疫的豚鼠的存活率明显高于 JE 疫苗。该结果为开发针对 JEV 的亚单位疫苗提供了有价值的信息。
