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关于固定在氯化锆上的丙酮粉和纯化漆树漆酶用于酚类氧化的研究。

Studies on acetone powder and purified rhus laccase immobilized on zirconium chloride for oxidation of phenols.

作者信息

Lu Rong, Miyakoshi Tetsuo

机构信息

Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki-shi 214-8571, Japan.

出版信息

Enzyme Res. 2012;2012:375309. doi: 10.1155/2012/375309. Epub 2012 Mar 25.

DOI:10.1155/2012/375309
PMID:22545205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3335288/
Abstract

Rhus laccase was isolated and purified from acetone powder obtained from the exudates of Chinese lacquer trees (Rhus vernicifera) from the Jianshi region, Hubei province of China. There are two blue bands appearing on CM-sephadex C-50 chromatography column, and each band corresponding to Rhus laccase 1 and 2, the former being the major constituent, and each had an average molecular weight of approximately 110 kDa. The purified and crude Rhus laccases were immobilized on zirconium chloride in ammonium chloride solution, and the kinetic properties of free and immobilized Rhus laccase, such as activity, molecular weight, optimum pH, and thermostability, were examined. In addition, the behaviors on catalytic oxidation of phenols also were conducted.

摘要

漆树漆酶是从中国湖北省建始地区的漆树（漆树科漆树属）渗出物制备的丙酮粉中分离纯化得到的。在CM-葡聚糖凝胶C-50色谱柱上出现两条蓝色条带，每条条带分别对应漆树漆酶1和漆树漆酶2，前者是主要成分，二者的平均分子量均约为110 kDa。将纯化的和粗制的漆树漆酶固定在氯化铵溶液中的氯化锆上，并检测游离和固定化漆树漆酶的动力学性质，如活性、分子量、最适pH值和热稳定性。此外，还研究了其对酚类的催化氧化行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/b62f0d7791e3/ER2012-375309.sch.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/8c7f77d287b4/ER2012-375309.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/ec1ae47db634/ER2012-375309.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/48fb534e9ae8/ER2012-375309.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/be57c4e49b09/ER2012-375309.sch.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/b62f0d7791e3/ER2012-375309.sch.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/8c7f77d287b4/ER2012-375309.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/ec1ae47db634/ER2012-375309.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/48fb534e9ae8/ER2012-375309.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/be57c4e49b09/ER2012-375309.sch.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb1/3335288/b62f0d7791e3/ER2012-375309.sch.003.jpg

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