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在污水和石化废水中生长的微藻的生长及重金属积累潜力

Growth and heavy metals accumulation potential of microalgae grown in sewage wastewater and petrochemical effluents.

作者信息

Ajayan K V, Selvaraju M, Thirugnanamoorthy K

机构信息

Environmental Science Division, Department of Botany, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India.

出版信息

Pak J Biol Sci. 2011 Aug 15;14(16):805-11. doi: 10.3923/pjbs.2011.805.811.

Abstract

Microalgae exhibit a number of heavy metal uptake process by different metabolism. In this study, the ability of microalgae for removal of heavy metal from wastewater was studied. Growth and biochemical contents of microalgae were determined by spectrophotometer. Heavy metal analysis of wastewater effluents were performed by atomic absorption spectrophotometer before and after treatment at laboratory scale. The growth of Scenedesmus bijuga and Oscillatoria quadripunctulata in sewage wastewater was higher than those grown in synthetic medium. Whereas, the growth of S. bijuga and O. quadripunctulata in sterilized petrochemical effluents was slightly lower than that grown in the standard synthetic medium. The chlorophyll, carotenoid and protein content of S. bijuga and O. quadripunctulata grown in sterilized sewage wastewater were higher than those grown in the standard medium. Similarly S. bijuga and O. quadripunctulata grown in sterilized petrochemical effluents showed lower contents of pigments and protein than those grown in sewage and synthetic medium. Heavy metals copper, cobalt, lead and zinc were removed by 37-50, 20.3-33.3, 34.6-100 and 32.1-100%, respectively from sewage wastewater and petrochemical effluent using Ocillatoria culture. The metal absorption by S. bijuga were (Cu, Co, Pb, Zn) 60-50, 29.6-66, 15.4-25 and 42.9-50%, respectively from sewage and petrochemical effluents. Both species showed high level of heavy metal removal efficiency and metal sorption efficiency of both microalgae depended on the type of biosorbent, the physiological status of the cells, availability of heavy metal, concentration of heavy metal and chemical composition of wastewater.

摘要

微藻通过不同的代谢过程表现出多种重金属吸收方式。在本研究中,对微藻从废水中去除重金属的能力进行了研究。微藻的生长和生化成分通过分光光度计测定。在实验室规模下,使用原子吸收分光光度计对处理前后的废水进行重金属分析。双对栅藻(Scenedesmus bijuga)和四 punctulata 颤藻(Oscillatoria quadripunctulata)在污水中的生长高于在合成培养基中的生长。而双对栅藻和四 punctulata 颤藻在灭菌的石化废水中的生长略低于在标准合成培养基中的生长。在灭菌污水中生长的双对栅藻和四 punctulata 颤藻的叶绿素、类胡萝卜素和蛋白质含量高于在标准培养基中生长的含量。同样,在灭菌石化废水中生长的双对栅藻和四 punctulata 颤藻的色素和蛋白质含量低于在污水和合成培养基中生长的含量。使用四 punctulata 颤藻培养物分别从污水和石化废水中去除重金属铜、钴、铅和锌的比例为 37 - 50%、20.3 - 33.3%、34.6 - 100%和 32.1 - 100%。双对栅藻对污水和石化废水中金属(铜、钴、铅、锌)的吸收分别为 60 - 50%、29.6 - 66%、15.4 - 25%和 42.9 - 50%。两种微藻都表现出较高的重金属去除效率,且两种微藻的金属吸附效率取决于生物吸附剂的类型、细胞的生理状态、重金属的可利用性、重金属浓度以及废水的化学成分。

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