Ford T C, Rickwood D
Department of Biology, University of Essex, Colchester, U.K.
J Immunol Methods. 1990 Dec 5;134(2):237-41. doi: 10.1016/0022-1759(90)90385-9.
This paper describes a radical new, one-step method for the routine isolation of the mononuclear cells from human blood in high yield and purity. In this procedure the blood sample is mixed with a Nycodenz separation medium prior to centrifugation. Erythrocytes and polymorphogranulocytes are pelleted during centrifugation leaving the mononuclear cells in the supernatant. The method can be used for a wide range of blood sample volumes and can be carried out with greater ease than the currently accepted methods.
本文描述了一种全新的一步法，用于从人血中常规分离出高产率和高纯度的单核细胞。在该程序中，血液样本在离心前与 Nycodenz 分离介质混合。红细胞和多形核粒细胞在离心过程中沉淀，单核细胞留在上清液中。该方法可用于多种血液样本体积，并且比目前公认的方法更易于操作。
