Willis R C, Seegmiller J E
J Cell Physiol. 1977 Dec;93(3):375-82. doi: 10.1002/jcp.1040930308.
The rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 can be inhibited greater than 95% by incubation of cell suspensions with 6-diazo-5-oxo-L-norleucine (DON). The inhibition persists for at least four hours after removal of DON from the cell suspension. The exposure of cells to DON ihibits over 95% of the glutaminase activity measured in lysates in the presence of either phosphate or maleate. Similarly, gamma-glutamyl transpeptidase, assayed with gamma-glutamyl-p-nitroanilide as substrate and glycyglycine as acceptor, is inhibited over 90%. DON-treated and control cells accumulated radioactive material from suspensions containing [14C]-L-glutamine at similar initial rates; the radioactive material accumulated by the DON-treated cells is all recoverable as glutamine while the radioactive material accumulated by untreated cells is principally recovered as glutamate.
通过将细胞悬液与6-重氮-5-氧代-L-正亮氨酸（DON）一起孵育，培养的人淋巴母细胞系WI-L2对谷氨酰胺的快速分解代谢可被抑制超过95%。从细胞悬液中去除DON后，这种抑制作用至少持续四小时。细胞暴露于DON会抑制在磷酸盐或马来酸盐存在下裂解物中测得的超过95%的谷氨酰胺酶活性。同样，以γ-谷氨酰-p-硝基苯胺为底物、甘氨酰甘氨酸为受体进行测定时，γ-谷氨酰转肽酶被抑制超过90%。用DON处理的细胞和对照细胞以相似的初始速率从含有[14C]-L-谷氨酰胺的悬液中积累放射性物质；用DON处理的细胞积累的放射性物质全部可作为谷氨酰胺回收，而未处理细胞积累的放射性物质主要作为谷氨酸回收。
