Institute of Molecular and Cell Biology, Proteos, Singapore.
Cell Biol Int. 2012 Aug 1;36(8):759-63. doi: 10.1042/CBI20110534.
Expression of PRL3 (phosphatase of regenerating liver 3) protein was examined with immunohistochemistry in 60 cases of ESCC (oesophageal squamous cell carcinoma) with matched lymph node metastasis (n = 40) and 6 cases of oesophageal adenocarcinoma. Its associations with PRL1 and clinicopathological parameters were analysed. The results showed the frequency of PRL3 protein expression was significantly higher in ESCC (39/60, 65%) than in normal oesophageal mucosa (0/20, P < 0.001); higher in ESCC with lymph node metastasis (30/40, 75%) than in ESCC without lymph node metastasis (9/20, P = 0.022), as well as higher in metastatic ESCC in lymph node (38/40, 95%) than in the primary ESCC (39/60, 65%, P < 0.001). PRL3 was expressed in 1 out of 6 oesophageal adenocarcinomas, but showed no nuclear staining of PRL1. Expression of PRL3 protein was positively associated with the grade and partially with the stage of ESCC. These results suggest that expression of PRL3 protein may be involved in the metastasis of ESCC and serve as a biomarker for prediction of ESCC metastasis.
PRL3（肝再生磷酸酶 3）蛋白的表达通过免疫组化在 60 例伴有淋巴结转移的 ESCC（食管鳞状细胞癌）病例（n=40）和 6 例食管腺癌病例中进行了检测。分析了其与 PRL1 及临床病理参数的相关性。结果显示，PRL3 蛋白在 ESCC 中的表达频率（39/60，65%）显著高于正常食管黏膜（0/20，P<0.001）；在伴有淋巴结转移的 ESCC 中（30/40，75%）高于无淋巴结转移的 ESCC（9/20，P=0.022），在淋巴结转移的 ESCC 中（38/40，95%）高于原发 ESCC（39/60，65%，P<0.001）。6 例食管腺癌中有 1 例表达 PRL3，但 PRL1 无核染色。PRL3 蛋白的表达与 ESCC 的分级呈正相关，与分期部分相关。这些结果表明，PRL3 蛋白的表达可能参与了 ESCC 的转移，并可作为 ESCC 转移预测的标志物。
