Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Leuk Res. 2012 Aug;36(8):998-1003. doi: 10.1016/j.leukres.2012.04.005. Epub 2012 Apr 30.
We analyzed minimal residual disease (MRD) by multidimensional flow cytometry (MFC) after allogeneic stem cell transplantation in 41 patients with acute myeloid leukemia (AML) (n=31) or acute lymphoblastic leukemia (ALL) (n=10). Aberrant antigen expression was compared with the results of quantitative PCR for WT1 mRNA (n=41) and leukemia-specific fusion transcripts (n=12; AML in seven, ALL in five). There was a significant correlation between detection of MRD by MFC and WT1 mRNA, as well as between MFC and fusion transcripts. Serial monitoring of MRD by the three techniques correlated in parallel to the clinical course in most of the patients, but three patients were only positive for WT1 during hematological remission. The overall survival time of patients with complete remission was significantly associated with the appearance of aberrant expression after transplantation. In conclusion, MFC is valuable for clinical management decisions after transplantation.
我们分析了 41 例接受异基因干细胞移植的急性髓系白血病（AML）（n=31）或急性淋巴细胞白血病（ALL）（n=10）患者的微小残留病（MRD）。异常抗原表达与 WT1 mRNA 的定量 PCR 结果（n=41）和白血病特异性融合转录本（n=12；AML7 例，ALL5 例）进行了比较。MFC 检测 MRD 与 WT1 mRNA 之间以及 MFC 与融合转录本之间存在显著相关性。三种技术的 MRD 连续监测与大多数患者的临床过程平行相关，但有 3 例患者在血液学缓解期间仅 WT1 呈阳性。完全缓解患者的总生存时间与移植后异常表达的出现显著相关。总之，MFC 对于移植后的临床管理决策具有重要价值。
