Chen Y H, MacGregor J P, Goldstein D A, Hall M R
J Virol. 1979 Apr;30(1):218-24. doi: 10.1128/JVI.30.1.218-224.1979.
Simian virus (SV40) nucleoprotein complexes containing circular supercoiled viral DNA were extracted from infected cells and purified by differential centrifugation. The protein content of these complexes was compared by electrophoresis on 15% acrylamide gels with the protein content of purified SV40 virions and with histones from virus-infected cells. The electrophoretic patterns of histones from each of the sources revealed several major differences. SV40 virions contained histones H3, H2B, H2A, and H4 but not H1. Nucleoprotein complexes and host cells contained all five major histone groups. Relative to cellular histones, virion and nucleoprotein complex histones were enriched 15 to 40% in histones H3 and H4. In addition to the major classes of histones, several subfractions of histones H1, H3, and H4 were observed in acrylamide gels of proteins from SV40 virions and viral nucleoprotein complexes. Acetate labeling experiments indicated that each subfraction of histones H3 and H4 had a different level of acetylation. The histones from SV40 virions and nucleoprotein complexes were acetylated to significantly higher levels than those of infected host cells. No apparent differences in phosphorylation of the major histone groups were observed.
从感染细胞中提取含有环状超螺旋病毒DNA的猴病毒（SV40）核蛋白复合物，并通过差速离心进行纯化。通过在15%丙烯酰胺凝胶上电泳，将这些复合物的蛋白质含量与纯化的SV40病毒粒子的蛋白质含量以及病毒感染细胞的组蛋白进行比较。来自每种来源的组蛋白的电泳图谱显示出几个主要差异。SV40病毒粒子含有组蛋白H3、H2B、H2A和H4，但不含有H1。核蛋白复合物和宿主细胞含有所有五个主要组蛋白组。相对于细胞组蛋白，病毒粒子和核蛋白复合物组蛋白中的H3和H4组蛋白富集了15%至40%。除了主要的组蛋白类别外，在SV40病毒粒子和病毒核蛋白复合物的蛋白质的丙烯酰胺凝胶中还观察到了组蛋白H1、H3和H4的几个亚组分。醋酸盐标记实验表明，组蛋白H3和H4的每个亚组分具有不同程度的乙酰化。SV40病毒粒子和核蛋白复合物的组蛋白乙酰化水平明显高于感染的宿主细胞。主要组蛋白组的磷酸化未观察到明显差异。
