痘苗病毒蛋白质组：痘苗病毒细胞内成熟病毒粒子中蛋白质的鉴定
Vaccinia virus proteome: identification of proteins in vaccinia virus intracellular mature virion particles.
作者信息
Chung Che-Sheng, Chen Chein-Hung, Ho Ming-Yi, Huang Cheng-Yen, Liao Chung-Lin, Chang Wen
机构信息
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
出版信息
J Virol. 2006 Mar;80(5):2127-40. doi: 10.1128/JVI.80.5.2127-2140.2006.
Abstract
Vaccinia virus is a large enveloped poxvirus with more than 200 genes in its genome. Although many poxvirus genomes have been sequenced, knowledge of the host and viral protein components of the virions remains incomplete. In this study, we used gel-free liquid chromatography and tandem mass spectroscopy to identify the viral and host proteins in purified vaccinia intracellular mature virions (IMV). Analysis of the proteins in the IMV showed that it contains 75 viral proteins, including structural proteins, enzymes, transcription factors, and predicted viral proteins not known to be expressed or present in the IMV. We also determined the relative abundances of the individual protein components in the IMV. Finally, 23 IMV-associated host proteins were also identified. This study provides the first comprehensive structural analysis of the infectious vaccinia virus IMV.
摘要
痘苗病毒是一种大型包膜痘病毒，其基因组中有200多个基因。尽管许多痘病毒基因组已被测序，但对病毒粒子的宿主和病毒蛋白成分的了解仍不完整。在本研究中，我们使用无胶液相色谱和串联质谱来鉴定纯化的痘苗细胞内成熟病毒粒子（IMV）中的病毒和宿主蛋白。对IMV中的蛋白质分析表明，它包含75种病毒蛋白，包括结构蛋白、酶、转录因子以及预测的在IMV中未被知晓表达或存在的病毒蛋白。我们还确定了IMV中各个蛋白质成分的相对丰度。最后，还鉴定出了23种与IMV相关的宿主蛋白。本研究首次对感染性痘苗病毒IMV进行了全面的结构分析。
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