State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, People's Republic of China.
Nanoscale. 2012 Jun 7;4(11):3551-7. doi: 10.1039/c2nr30233k. Epub 2012 May 2.
Silica-encapsulated luminescent lanthanide nanoparticles have shown great potential as biolabels for various time-gated luminescence bio-detections in recent years. The main problem of these nano-biolabels is their short excitation wavelengths within the UV region. In this work, a new type of silica-encapsulated luminescent europium nanoparticle, with a wide excitation range from UV to visible light in aqueous solutions, has been prepared using a conjugate of (3-isocyanatopropyl)triethoxysilane bound to a visible light-excited Eu(3+) complex, 2,6-bis(1',1',1',2',2',3',3'-heptafluoro-4',6'-hexanedion-6'-yl)-dibenzothiophene-Eu(3+)-2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine (IPTES-BHHD-Eu(3+)-BPT conjugate), as a functionalized precursor. The nanoparticles, which are prepared by the copolymerization of the IPTES-BHHD-Eu(3+)-BPT conjugate, tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia, are monodisperse, spherical and uniform in size. Their diameter is 42 ± 3 nm and they are strongly luminescent with a wide excitation range from UV to ∼475 nm and a long luminescence lifetime of 346 μs. The nanoparticles were successfully used for streptavidin labeling and the time-gated luminescence imaging detection of two environmental pathogens, cryptosporidium muris and cryptosporidium parvium, in water samples. The results demonstrated the practical utility of the new nanoparticles as visible light-excited biolabels for time-gated luminescence bioassay applications.
近年来，硅壳包裹的发光镧系纳米粒子作为各种时间门控荧光生物检测的生物标记物显示出巨大的潜力。这些纳米生物标记物的主要问题是它们的激发波长较短，处于紫外区域。在这项工作中，我们使用（3-异氰酸丙基）三乙氧基硅烷与可见光谱激发的 Eu(3+) 配合物 2,6-双（1'，1'，1'，2'，2'，3'，3'-七氟-4'，6'-己二酮-6'-基）-二苯并噻吩-Eu(3+)-2-（N，N-二乙基苯胺-4-基）-4,6-双（吡唑-1-基）-1,3,5-三嗪（IPTES-BHHD-Eu(3+)-BPT 配合物）的偶联物作为功能化前体，制备了一种新型硅壳包裹的发光铕纳米粒子。该纳米粒子通过在 Triton X-100、正辛醇、环己烷和水组成的油包水反微乳液中，共聚 IPTES-BHHD-Eu(3+)-BPT 配合物、正硅酸乙酯和（3-氨丙基）三乙氧基硅烷，并在氨水溶液的存在下制备得到。这些纳米粒子单分散性好，呈球形且尺寸均匀，直径为 42±3nm，具有从紫外到约 475nm 的宽激发范围和 346μs 的长荧光寿命，发光强度很高。这些纳米粒子成功地用于链霉亲和素标记，并在水样中对两种环境病原体微小隐孢子虫和微小隐孢子虫进行了时间门控荧光成像检测。结果表明，这些新纳米粒子作为可见光激发的生物标记物，在时间门控荧光生物分析应用中具有实际应用价值。
