State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China.
Talanta. 2013 Apr 15;108:143-9. doi: 10.1016/j.talanta.2013.02.065. Epub 2013 Mar 7.
By using a visible-light-excited ternary Eu(3+) complex, BHHBCB-Eu(3+)-BPT (BHHBCB: 1,2-bis[4'-(1",1",1",2",2",3",3"-heptafluoro-4″,6″-hexanedion-6″-yl)-benzyl]-4-chlorosulfobenzene; BPT: 2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine), as a luminophore, two kinds of novel visible-light-excited europium materials, the silica-encapsulated BHHBCB-Eu(3+)-BPT (Eu@SiO2) nanoparticles and BHHBCB-Eu(3+)-BPT-conjugated bovine serum albumin (BSA-BHHBCB-Eu(3+)-BPT), were prepared for biolabeling and time-resolved luminescence cell imaging applications. The Eu@SiO2 nanoparticles, prepared by the copolymerization of 3-aminopropyl(triethoxy)silane-BHHBCB-Eu(3+)-BPT conjugate, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion, are monodispersed, spherical and uniform in size, and strongly luminescent with an excitation peak at ≈ 400 nm and a long luminescence lifetime of 346 μs. The BSA-BHHBCB-Eu(3+)-BPT, prepared by covalent binding of BHHBCB-Eu(3+)-BPT to BSA, shows also strong visible-light-excited luminescence with a excitation peak at ≈ 400 nm and a long luminescence lifetime of 402μs. The two materials were used for labeling transferrin and folic acid. Using the time-resolved luminescence imaging of living HeLa cells, the cell-surface receptors of transferrin and folic acid were successfully visualized by the prepared biolabels based on the ligand-receptor affinity binding interaction. The results demonstrated the feasibility of the new materials as visible-light-excited biolabels for the time-resolved luminescence cell imaging.
采用可见光照激发的三元铕(III)配合物 BHHBCB-Eu(3+)-BPT（BHHBCB：1,2-双[4'-(1",1",1",2",2",3",3"-七氟-4″,6″-己二酮-6″-基)-苄基]-4-氯磺酰基苯；BPT：2-(N,N-二乙基苯胺-4-基)-4,6-双(吡唑-1-基)-1,3,5-三嗪）作为发光体，制备了两种新型可见光照激发的铕材料，即硅烷封端的 BHHBCB-Eu(3+)-BPT（Eu@SiO2）纳米粒子和 BHHBCB-Eu(3+)-BPT 偶联牛血清白蛋白（BSA-BHHBCB-Eu(3+)-BPT），用于生物标记和时间分辨荧光细胞成像应用。Eu@SiO2 纳米粒子通过 3-氨丙基（三乙氧基）硅烷-BHHBCB-Eu(3+)-BPT 配合物、游离 3-氨丙基（三乙氧基）硅烷和正硅酸乙酯在油包水反相微乳液中的共聚反应制备，呈单分散、球形且粒径均匀，激发峰约为 400nm，荧光寿命为 346μs。BSA-BHHBCB-Eu(3+)-BPT 通过 BHHBCB-Eu(3+)-BPT 与 BSA 的共价结合制备，也具有较强的可见光照激发发光，激发峰约为 400nm，荧光寿命为 402μs。这两种材料被用于标记转铁蛋白和叶酸。通过活 HeLa 细胞的时间分辨荧光成像，基于配体-受体亲和结合相互作用，成功地利用制备的生物标记物可视化了转铁蛋白和叶酸的细胞表面受体。结果表明，这些新材料作为可见光照激发生物标记物用于时间分辨荧光细胞成像具有可行性。
