Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland; Department of Mechanical & Biomedical Engineering, National University of Ireland Galway, Galway, Ireland.
J Tissue Eng Regen Med. 2014 Mar;8(3):233-41. doi: 10.1002/term.1522. Epub 2012 May 2.
Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra-structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications.
多糖经常被纳入组织工程应用的支架中，以改善其机械和生物学性能。我们评估了 Ficoll®支架对胶原蛋白膜的影响，胶原蛋白膜广泛用于软组织和硬组织修复。为了避免与化学试剂相关的细胞毒性问题，评估了天然交联剂京尼平的影响。有和没有 Ficoll 的超结构水平胶原蛋白膜显示出精细的纤维状结构，而京尼平交联膜显示出粗糙的纤维状和部分结节状结构。相比之下，戊二醛交联膜失去了纤维状图案。交联显著增加了变性温度（p<0.001）、应力（p<0.0001）和断裂力（p<0.0001）。与任何其他支架相比，胶原蛋白/Ficoll 和胶原蛋白/Ficoll/京尼平膜显示出最高的 WI38 成纤维细胞附着（p<0.003），并且与其他支架相比，WI38 成纤维细胞代谢活性显著更高（p<0.001）。到第 6 天，胶原蛋白/Ficoll/京尼平膜还诱导了比其他支架更高和更对齐的纤维连接蛋白基质沉积。总的来说，这项研究表明胶原蛋白/Ficoll/京尼平适用于组织工程应用。
