Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea.
Arch Pharm Res. 2012 Mar;35(4):739-46. doi: 10.1007/s12272-012-0419-x. Epub 2012 May 3.
Amomum compactum is commonly used in Korean traditional medicine. In this study, we demonstrate that A. compactum ethanolic extract (ACEE) has anti-inflammatory effects in a lipopolysaccharide-induced RAW 264.7 cell model of inflammation. In this system, ACEE prominently inhibited the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, and inhibited the protein expression of inducible nitric oxide synthase and cyclooxygenase-2. Furthermore, ACEE treatment inhibited the translocation of nuclear factor-kappaB (NF-κB) and the degradation of inhibitory factor-kappaB alpha, but enhanced the expression of heme oxygenase (HO)-1 and the nuclear translocation of nuclear factor-erythroid 2 (Nrf2). Treatment with tin protoporphyrin IX dichloride (SnPP), a selective HO-1 inhibitor, reversed the ACEE-induced suppression of NO production, suggesting that the induction of HO-1 is involved in the suppression of NO, TNF-α, and IL-6 production by ACEE. Taken together, these results suggest that ACEE have anti-inflammatory effects occurring through HO-1 induction, which leads to suppression of the blocking NF-κB.
密砂仁常被用于韩国传统医学中。在本研究中，我们证实密砂仁乙醇提取物（ACEE）在脂多糖诱导的 RAW 264.7 细胞炎症模型中具有抗炎作用。在该系统中，ACEE 显著抑制一氧化氮（NO）、前列腺素 E（2）（PGE（2））、白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α的产生，并抑制诱导型一氧化氮合酶和环氧化酶-2 的蛋白表达。此外，ACEE 处理抑制核因子-κB（NF-κB）的易位和抑制因子-κBα的降解，但增强血红素加氧酶（HO-1）的表达和核因子-红细胞 2（Nrf2）的核易位。用锡原卟啉 IX 二氯化物（SnPP）处理，一种选择性 HO-1 抑制剂，逆转了 ACEE 诱导的 NO 产生抑制，表明 HO-1 的诱导参与了 ACEE 抑制 NO、TNF-α 和 IL-6 的产生。综上所述，这些结果表明 ACEE 具有抗炎作用，其发生机制是通过诱导 HO-1 来抑制 NF-κB 的阻断。
