Blain L, Lachapelle P, Molotchnikoff S
Département des Sciences Biologiques, Université de Montréal, Québec, Canada.
Neurotoxicol Teratol. 1990 Nov-Dec;12(6):633-6. doi: 10.1016/0892-0362(90)90076-o.
The effects of acute exposure to trichloroethylene (TCE) on the electroretinogram (ERG) and oscillatory potentials (OPs) were studied on adult New Zealand rabbits anesthetized with urethane. The ERG and OPs were recorded under photopic conditions with a speculum electrode, with pupils fully dilated and the cornea anesthetized. Emulsified (Intralipid) TCE was given intravenously in three consecutive injections of equal volume for a total dose varying between 24 and 85 mg/kg. Averaged ERG and OPs (50 sweeps) were taken up to 250 minutes after the last TCE injection. Our results indicated that, irrespective of the dose used, there was a significant (p less than 0.001) reduction (average: 50%) in the amplitude of the b-wave of the ERG following TCE injection. In contrast, the OPs showed a differential effect. At low doses, OP4 showed a significant (p less than 0.001) reduction resulting in some cases to its complete extinction, while at higher dose there is a marked increase in amplitude. Our results would suggest that the OPs are a better retinal potential to assess the level of TCE toxicity than is the b-wave of the ERG.
研究了急性暴露于三氯乙烯（TCE）对用氨基甲酸乙酯麻醉的成年新西兰兔视网膜电图（ERG）和振荡电位（OPs）的影响。在明视条件下，使用窥镜电极记录ERG和OPs，使瞳孔充分扩张并麻醉角膜。将乳化的（脂质乳剂）TCE以等体积连续三次静脉注射给药，总剂量在24至85mg/kg之间变化。在最后一次注射TCE后长达250分钟记录平均ERG和OPs（50次扫描）。我们的结果表明，无论使用何种剂量，注射TCE后ERG的b波振幅均显著降低（p<0.001）（平均降低50%）。相比之下，OPs表现出不同的效应。在低剂量时，OP4显著降低（p<0.001），在某些情况下导致其完全消失，而在高剂量时，振幅显著增加。我们的结果表明，与ERG的b波相比，OPs是评估TCE毒性水平更好的视网膜电位。
